Effect of ACTN4 on cell proliferation of esophageal squamous cell carcinoma by targeting NDUFV1
10.19405/j.cnki.issn1000-1492.2022.11.018
- Author:
Cheng Feng
1
;
Xiaomin Cheng
1
;
Yiqing Xie
2
;
Liping Kang
1
;
Zhangran Sun
1
;
Xu Fan
1
;
Huiwu Geng
1
;
Xiaoying Liu
1
Author Information
1. Dept of Cell Biology，School of Life Sciences，Anhui Medical University，Hefei 230032
2. School of Clinical Medicine，Bengbu Medical College，Bengbu 233030
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
alpha-actinin-4;
cell proliferation;
NADH ∶ ubiquinone oxi- doreductase core subunit V1
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1790-1795
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of alpha-actinin-4 (ACTN4) and the effect on cell proliferation in esophageal squamous cell carcinoma (ESCC) ．
Methods :The expression of ACTN4 in ESCC tissues and paired normal tissues was detected by immunohistochemistry，and the correlation between ACTN4 and the clinicopathologi- cal features was analyzed statistically．The ACTN 4 shRNA lentiviral plasmids were constructed ，and the stable ECA109 strain with ACTN4 shRNA knockdown was established using lentivirus packaging technology．The knock- down efficiency on protein level was checked by Western blot，and cell proliferation was detected by colony forma- tion assays．The downstream target proteins were validated in ESCC cell line ECA109 based on the previous pro- teomics analyses in melanoma cell line A375 with or without ACTN4 shRNA knockdown.
Results :The expression of ACTN4 in ESCC tissues was significantly higher than that of normal tissues．ACTN4 shRNA stable knockdown ECA109 cell strains were successfully constructed． The results of colony formation assays showed that ACTN4 knockdown inhibited the cell proliferation and down-regulated NADH ∶ Ubiquinone oxidoreductase core subunit V1 ( NDUFV1) protein expression in ECA109 cells．
Conclusion :Upregulation of ACTN4 in ESCC cells promotes the cell proliferation and enhances the protein expression of NDUFV1 .
- Full text:2024072521211040315ACTN4通过靶向NDUF...鳞状细胞癌的细胞增殖的影响_冯成.pdf
