Expression and diagnostic value of plasma exosomes miR - 26a－5p and miR - 151a－3p in tuberculosis
10.19405/j.cnki.issn1000-1492.2022.12.022
- Author:
Zhen1 Feng
1
;
Bo1 Zhang
1
;
Siqi1 Deng
1
;
Guomin1 Ye
1
;
Hui1 Zhang
1
;
Wanjiang1 Zhang
1
;
Jiangdong1 Wu
1
;
Weimin2 Li
2
Author Information
1. Key Laboratory of Xinjiang Endemic and Ethnic Diseases Cooperated by Education Ministry with Xinjiang Province，Shihezi University，Shihezi 832002
2. Key Laboratory of Xinjiang Endemic and Ethnic Diseases Cooperated by Education Ministry with Xinjiang Province，Shihezi University，Shihezi 832002;National Tuberculosis Clinical Laboratory，Beijing Chest Hospital，Capital Medical University，Beijing 101100
- Publication Type:Journal Article
- Keywords:
tuberculosis;
exosome;
miR-26a-5p;
miR-151a-3p;
verification
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1979-1984
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the differential expression and diagnostic value of miR-26a-5p and miR-151a-3p in plasma exosomes of tuberculosis.
Methods :Differentially expressed miRNAs ( miR-26a-5p and miR-151a-3p) in tuberculosis exosomes were identified through bioinformatics website．Furthermore，70 tuberculosis patients (tuber- culosis group) and 58 healthy subjects ( healthy control group) were selected for clinical validation : Plasma exo- somes，extracted by differential centrifugation，were identified by Western blot，transmission electron microscopy， nanoparticle tracking analysis．The expression levels of miR-26a-5p and miR-151a-3p in plasma exosome were de- tected by RT-qPCR in the tuberculosis group and the control group．The diagnostic value of miR-26a-5p and miR- 151a-3p in tuberculosis was evaluated by ROC curve.
Results :The characteristics of exosomes were identified by transmission electron microscopy，nanoparticle tracking analysis and Western blot，which showed that plasma exo- somes were extracted successfully．In clinical validation，the internal and external parameters were calibrated sim- ultaneously，the expression level of miR-26a-5p in plasma exosome of patients with tuberculosis was lower than that in control group (P＜0. 000 1) ，and the expression level of miR-151a-3p in plasma exosome of patients with tuber- culosis was higher than that in control group (P＜0. 000 1) ，which were consistent with the bioinformatics results. As a diagnostic marker，taking internal reference as the benchmark，the AUC of plasma exosome miR-26a-5p and miR-151a-3p for tuberculosis differentiation was 0. 872 and 0. 709，the AUC of the combined miR-26a-5p and miR- 151a-3p was 0. 915 (P ＜0. 000 1 ) ． Taking external reference as the benchmark ，the AUC of plasma exosomes miR-26a-5p and miR-151a-3p were 0. 829 and 0. 854 respectively ，the AUC of the combined miR-26a-5p and miR-151a-3p was 0. 911 (P＜0. 000 1) ．
Conclusion :The expression level of plasma exosome-derived miR-26a- 5p decreases in tuberculosis patients and the expression levelof miR-151a-3p increases，and the clinical diagnostic efficiency of bothis high，which may be a potential biomarker for the diagnosis of tuberculosis.
- Full text:2024072222160094017血浆外泌体miR-26a-...在结核病中的表达及诊断价值_冯真.pdf
