Effects of Celastrus orbiculatus extract on gastric organoids and expression of E －caherin in mice
10.19405/j.cnki.issn1000-1492.2023.03.020
- Author:
Fangyuan Zhu
1
;
Xiaoze Zhang
1
;
Yanqing Liu
2
;
Ping Li
1
;
Yaodong Zhu
1
Author Information
1. Dept of Chinese Integrative Medicine Oncology，The First Affiliated Hospital of Anhui Medical University， Hefei 230022
2. College of Medicine，Yangzhou University，Yangzhou 225003
- Publication Type:Journal Article
- Keywords:
Celastrus orbiculatus extract;
gastric organoids;
mice;
E-cadherin
- From:
Acta Universitatis Medicinalis Anhui
2023;58(3):471-476
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Celastrus orbiculatus extract ( COE) on the growth of gastric organoids and the expression of E-cadherin in gastric epithelial cells of mice．
Methods:The gastric pylorus of 8-week-old C57 mice was isolated and cultured into gastric organoids．The dynamic changes of gastric organoid formation were observed under light microscope ; the intercellular structure of gastric epithelium was observed by HE staining ; the expression of epithelial-cadherin E-cadherin in gastric epithelial cells was observed by immunofluorescence staining．After passage to the third-generation ，the organoids were treated with different concentrations of COE (0，5 ，10，20 μg/ ml) ，the organoids were collected ，their numbers were counted ，their diameters were measured，their cellular activities were measured by methyl thiazolyl tetrazolium( MTT) colorimetry，and Western blot was used to detect the expression of E-cadherin in organoids after COE treatment.
Results :At 24 to 48 h， cystlike structures were formed and three-dimensional cell clusters with cystic gland-like central structure appeared， and gradually budding and forming gastric organoids after 72 h，suggesting that the organoids were successfully constructed．The epithelial cell marker E-cadherin was expressed in the organoid，which further confirmed the formation of organoid．Compared with the control group，the number and diameter of gastric organoids in the COE group significantly increased，cell activity was significantly enhanced (P＜0. 05) ，and the expression of E-cadherin increased with the increase of COE dose (P＜0. 01) ．
Conclution :Low dose COE can promote the expression of E-cadherin and the growth and formation of organoids，which may affect the repair of gastric mucosa injury.
- Full text:2024071711240084379南蛇藤提取物对小鼠胃类器官...-caherin表达的影响_朱方圆.pdf
