ST3GAL5 inhibited malignant biological behavior of bladder cancer by activation of the PPAR pathway and inhibition of the PI3K/AKT pathway
10.19405/j.cnki.issn1000-1492.2023.09.005
- Author:
Minghui Tan
;
Yu Hao
1
;
Zhao Ni
1
;
Qiang Li
1
;
Qinzhang Wang
1
;
Song Ouyang
1
Author Information
1. Dept of Urology , The First Afiliated Hospital , School of Medicine , Shihezi University , Shihezi 832000
- Publication Type:Journal Article
- Keywords:
bladder cancer;
ST3GAL5;
TCGA;
GEO;
CCLE;
Western blot;
KEGG
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1458-1466
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To the effects and potential mechanisms of ST3GAL5 on biological behaviors of Bladder
Urothelial Carcinoma(BLCA) .
Methods : Differentially expressed genes related to bladder cancer were identified
using microarray analysis . Suitable bladder cancer cell lines were then screened . In vitro experimental measurements , including CCK8 , EdU , colony formation assays , transwell migration , flow cytometry apoptosis experiments , scratch assay , were used to evaluate the effects of ST3GAL5 on biological behaviors of BLCA . ST3GAL5 gene Kyoto Encyclopedia of Genes and Genomes ( KEGG) , gene set enrichment analysis ( GSEA) were analyzed using The Cancer Genome Atlas (TCGA) database . Finally , Western blot technology was used to verify the classical proliferation and metastasis related pathway factors .
Results : The combination of bioinformatics analyses and experimental
measurements demonstrate that ST3GAL5 expression is aberrantly down⁃regulated in human cell lines of BLCA . Through Cancer Cell Line Encyclopedia (CCLE) database , HT⁃1376 cell lines were successfully screened for vitro test . Upregulation of ST3GAL5 was found to suppress the malignant biological behaviour of bladder cancer. GSEA enrichment analyses exhibited that ST3GAL5 and its co⁃expressed genes inhibited cell proliferation , invasion and metastasis of bladder urothelial carcinoma by activation of the PPAR pathway and inhibition of the PI3K/AKT pathway . The results of Western blot experiments verified that the key proteins of the PPAR signaling pathway showed a
significant increase and the key proteins of the PI3K/AKT signaling pathway showed a significant decrease ( P <0. 05) after ST3GAL5 overexpression in bladder cancer.
Conclusion :ST3GAL5 gene might act as an oncogenic suppressor gene in bladder cancer , possibly inhibit the proliferation , invasion and metastasis of bladder cancer cells by activating the PPAR signaling pathway and inhibiting related molecules in the PI3K/AKT signaling pathway .
- Full text:2024071617522865442ST3GAL5通过激活PP...抑制膀胱癌的恶性生物学行为_谭明辉.pdf
