Bufalin induces apoptosis of HCT116 cells by activating endoplasmic reticulum stress pathway
10.19405/j.cnki.issn1000-1492.2023.02.018
- Author:
Jing Shang
1
;
Zongheng Li
1
;
Qi Xia
2
;
Donghao Tang
1
;
Jia Chen
2
;
Zeting Yuan
2
;
Peihao Yin
3
Author Information
1. Shanghai Putuo Central School of Clinical Medicine，Anhui Medical University，Shanghai 200062 ;The Fifth School of Clinical Medicine，Anhui Medical University，Hefei 230032 ;Interventional Cancer Institute of Chinese Integrative Medicine，Shanghai University of Traditional Chinese Medicine，Shanghai 200062
2. Dept of General Surgery， Putuo Hospital，Shanghai University of Traditional Chinese Medicine，Shanghai 200062;Interventional Cancer Institute of Chinese Integrative Medicine，Shanghai University of Traditional Chinese Medicine，Shanghai 200062
3. Shanghai Putuo Central School of Clinical Medicine，Anhui Medical University，Shanghai 200062 ;The Fifth School of Clinical Medicine，Anhui Medical University，Hefei 230032 ; Dept of General Surgery， Putuo Hospital，Shanghai University of Traditional Chinese Medicine，Shanghai 200062; Interventional Cancer Institute of Chinese Integrative Medicine，Shanghai University of Traditional Chinese Medicine，Shanghai 200062
- Publication Type:Journal Article
- Keywords:
bufalin;
endoplasmic reticulum stress;
cell apoptosis;
colorectal cancer
- From:
Acta Universitatis Medicinalis Anhui
2023;58(2):274-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the effect of bufalin on the proliferation and apoptosis of human colorectal cancer cell line HCT116，and to explore the role of endoplasmic reticulum stress ( ERS) in this process．
Methods :The effect of bufalin on the proliferation of HCT116 cells was determined by CCK-8 assay．After HCT116 cells were treated with different concentrations of bufalin for 48 hours，cell apoptosis was detected by Annexin V / PI assay， and the expression of apoptosis-related proteins Bax and Bcl-2 was detected by Western blot．At the same time，the expression of ERS-related proteins glucose regulated protein 78 ( GRP78) ，phosphorylated protein kinase R like endoplasmic reticulum kinase ( p-PERK) ，eukaryotic translation initiation factor 2 α ( eIF2 α) ，phosphorylated eukaryotic translation initiation factor 2 α (p-eIF2 α) and C / EBP homologous protein ( CHOP) was detected by Western blot．HCT116 cells were divided into control group，bufalin group and combination group (bufalin + 4-phenylbutyric acid) ，and the expression of apoptosis-related proteins Bax and Bcl-2 was observed by Western blot.
Results:CCK-8 assay showed that bufalin could inhibit the proliferation of HCT116 cells．Apoptosis assay showed that bufalin could induce apoptosis of HCT116 cells．The results of Western blot showed that bufalin could up-regulate the expression of pro-apoptotic protein Bax and down-regulate the expression of anti-apoptotic protein Bcl-2．It could also induce ERS and activate PERK / eIF2 α/ CHOP pathway．When bufalin combined with 4-phenylbutyric acid，the apoptosis-promoting effect of bufalin was inhibited．
Conclusion:Bufalin can effectively inhibit the prolif- erative activity and induce apoptosis of HCT116，which is achieved to some extent by activating ERS.
- Full text:2024071422161071770蟾毒灵通过激活内质网应激通路诱导HCT116细胞凋亡_尚靖.pdf
