The effect of MSR1 on the secretion of inflammatory factors and lipid accumulation in silicosis mice
10.19405/j.cnki.issn1000-1492.2023.11.021
- Author:
Yi Liu
1
;
Jincheng Li
1
;
Yuhui Zhou
1
;
Hailan He
1
;
Lingli Guo
2
Author Information
1. School of Public Health ,North China University of Science and Technology, Tangshan 063210
2. School of Public Health ,North China University of Science and Technology, Tangshan 063210 ; Hebei Key Laboratory of Organ Fibrosis , Tangshan 063210
- Publication Type:Journal Article
- Keywords:
silicosis;
inflammatory response;
lipid metabolism;
class A scavenger receptor;
macrophage;
MSR1
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1928-1933
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of class A scavenger receptor 1(MSR1) in the lungs of silico⁃
sis mice and its role in inflammation and lipid metabolism mediated by mouse mononuclear macrophages
(RAW264. 7) .
Methods : 24 C57BL/6 male mice were randomly divided into control group , exposed 7 d group , exposed 14 d group , exposed 28 d group , with 6 mice in each group. RAW264. 7 cells were divided into control
group , siRNA⁃MSR1 group , SiO2 group and siRNA⁃MSR1 + SiO2 stimulation group. The pathological changes of lung tissue in mice were observed by HE and VG staining. Lipid accumulation was observed under oil red O staining microscope. Immunohistochemical staining (IHC) was used to detect the expression and localization of MSR1 . The expression of MSR1 , tumor necrosis factor (TNF) Ⅳα , interleukin (IL) Ⅳ6 and IL⁃1β were detected by Western blot.
Results :Compared with the control group , HE and VG staining results showed that inflammatory cells gathered and collagen distribution increased in the lung tissue of silicosis mice. Oil red O staining showed that a large number of orange⁃red lipid droplets appeared in the lung tissue of mice. IHC results showed that the expression of MSR1 was up⁃regulated in silicosis inflammation stage. Western blot results showed that the expression of MSR1, TNF⁃α , IL⁃6 and IL⁃1β was up⁃regulated in silicosis inflammation stage (P < 0. 05) . The expression of MSR1 in the SiO2 cell stimulation group was up⁃regulated ( P < 0. 05 ) , and the expression of MSR1 in the siRNA⁃MSR1 group decreased (P < 0. 05) , and lipid droplets also appeared in the SiO2 cell stimulation group. The accumulation of lipid droplets in siRNA⁃MSR1 + SiO2 stimulation group was lower than that in SiO2 group (P < 0. 01) . ELISA results showed that the expression of TNF⁃α , IL⁃6 and IL⁃1β in SiO2 cell stimulation group was up⁃regulated ( P <0. 05) . Compared with SiO2 group , the expression of TNF⁃α , IL⁃6 and IL⁃1β in siRNA⁃MSR1 + SiO2 stimulation group was down⁃regulated (P < 0. 05) .
Conclusion :MSR1 is involved in the regulation of lipid components and the release of inflammatory factors in lung tissue and cells of silicosis mice. Inhibition of MSR1 expression can an⁃ tagonize the inflammatory response and abnormal lipid accumulation in macrophages. MSR1 may be a potential therapeutic target for future intervention in the progression of silicosis.
- Full text:2024071211260687146MSR1对矽肺小鼠脂质蓄积和炎性因子分泌的影响研究_刘艺.pdf
