Expression and clinical significance of FAM83H-AS1 in breast cancer based on bioinformatics analysis
10.3969/j.issn.1673-9701.2024.09.001
- VernacularTitle:基于生物信息学分析FAM83H-AS1在乳腺癌中的表达及临床意义
- Author:
Na LI
1
;
Jun YUAN
1
;
Anzhi ZHANG
1
;
Chuling HU
2
;
Maoyi XU
3
Author Information
1. Department of Pathology, Jiaxing Maternity and Child Health Hospital, Affiliated Women and Children’s Hospital of Jiaxing University, Jiaxing 314051, Zhejiang, China
2. Department of Pharmacy, Jiaxing Maternity and Child Health Hospital, Affiliated Women and Children’s Hospital of Jiaxing University, Jiaxing 314051, Zhejiang, China
3. Department of Oncology, the First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China
- Publication Type:Journal Article
- Keywords:
FAM83H-AS1;
Breast cancer;
Immune checkpoint;
Tumor microenvironment;
Tumor mutation burden
- From:
China Modern Doctor
2024;62(9):1-6,21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression and clinical treatment and prognosis assessment significance of FAM83H-AS1 in breast cancer by bioinformatics analysis.Methods The expression of FAM83H-AS1 in various cancer types was analyzed in the Cancer Genome Atlas(TCGA)database by R language.Additionally the expression in breast cancer was examined by Gene Expression Profiling Interactive Analysis(GEPIA)online database.Survival data were downloaded from TCGA to analyze the correlation between FAM83H-AS1 expression levels and prognosis among breast cancer patients.GEPIA and Kaplan-Meier Plotter were used for dual verification.Clinical information was obtained from TCGA for further analysis on the relationship between FAM83H-AS1 and clinical characteristics.And the relationship between FAM83H-AS1 and tumor microenvironment,immunodetection point-related genes and tumor mutation burden(TMB)was analyzed using R language.Gene set enrichment analysis(GSEA)was performed.Results The expression of FAM83H-AS1 was abnormal in many cancers,particularly exhibiting a significant increase in breast cancer.High expression of FAM83H-AS1 is associated with significantly reduced overall survival in breast cancer patients.Furthermore,the expression level of FAM83H-AS1 varies among breast cancer patients with different clinical stages.FAM83H-AS1 exhibits negative correlation with stromal cell score and immune cell score in breast cancer samples,and correlated with immune detection point-related gene.TMB radar map results suggest that the expression of FAM83H-AS1 in breast cancer is positively correlated with tumor mutational burden.GSEA revealed a positive correlation between the expression of FAM83H-AS1 and the function of mismatch repair.Conclusion FAM83H-AS1 is highly expressed in breast cancer,and is related to poor prognosis.Its expression correlates with clinicopathological stage,tumor microenvironment and TMB of breast cancer patients.Furthermore,FAM83H-AS1 exhibits associations with certain immune checkpoint-related genes and mismatch repair genes.These findings provide a important theoretical basis for clinical treatment,prognosis prediction and development of gene-target drugs.
