Effect of electroacupuncture on expression of V2R,AQP2 and ENaC-α in cochlea of guinea pigs with endolymphatic hydrops
10.3969/j.issn.1673-9701.2023.35.013
- VernacularTitle:电针对膜迷路积水豚鼠耳蜗V2R、AQP2、ENaC-α表达的影响
- Author:
Liyuan JIANG
1
;
Tiantian YE
;
Junwen YANG
Author Information
1. 杭州市中医院针灸康复科,浙江杭州 310007
- Keywords:
Electroacupuncture;
Endolymphatic hydrops;
Vasopressin type 2 receptor;
Aquaporin 2;
Epithelial sodium channel-α
- From:
China Modern Doctor
2023;61(35):54-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electroacupuncture(EA)on the expression of vasopressin type 2 receptor(V2R),aquaporin-2(AQP2)and epithelial sodium channel-α(ENaC-α)in guinea pigs with arginine vasopressin(AVP)-induced endolymphatic hydrops(EH).Methods Male albino guinea pigs were randomly divided into normal control,EH model and EA groups(n=12 guinea pigs in each group).The EH model was established by continuous injection intraperitoneally of AVP,4μg/(kg·d)for 7 days,and then 6μg/(kg·d)for 3 days,totally 10 days.EA(100Hz,1mA)was applied to Baihui(GV 20)and unilateral Tinggong(SI 19)for 20 min,once a day for continuous 10 days.Cochlear hydrops severity was measured using hematoxylin-eosin(HE)staining,and then the ratio of scala media(SM)area to SM + scala vestibuli(SV)area(R value)was calculated.The expression level of V2R,AQP2 and ENaC-α in the cochlea was detected by western blot.Results Following modeling,R value,the V2R,AQP2 and ENaC-α protein expression were significantly increased in comparison with the normal group(all P<0.01).After EA intervention,R value,the V2R,AQP2 and ENaC-α protein expression were notably down-regulated in comparison with the model group(all P<0.01).Conclusion EA can relieve the cochlear hydrops of EH guinea pigs,which may be related to its effect in down-regulating the expression of V2R,AQP2 and ENaC-α protein in the cochlea.
