Correlation analysis between the expression of CD47 and the degree of cervical lesions
10.19405/j.cnki.issn1000-1492.2023.01.012
- Author:
Yuting Zhu
1
;
Shuguang Zhou
1
;
Lu Tian
1
;
Jie Ying
1
;
Hui Yao
1
;
Shunxia Chen
1
;
Guo Chen
1
Author Information
1. Dept of Gynecology, The Afiliated Maternal and Child Health Hospital of Anhui Medical University, Hefei 230001
- Publication Type:Journal Article
- Keywords:
CD47;
cervical cancer;
cervical lesions;
E6/E7 mRNA
- From:
Acta Universitatis Medicinalis Anhui
2023;58(1):67-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation between CD47 and cervical lesions.
Methods:The expression and overall survival of CD47 in cervical cancer was analyzed using TCGA and TCGA Target GTEx databases. Based on GEO databases , the expression of CD47 was analyzed between cervical sample infected by HPV 16/18 and nonHPV. The differences of CD47 expression in the degrees of cervical lesions infected by HPV 16/18 were compared.The patholgical sections of colposcopy biopsy came from 320 patients who were admitted cervical cancer screening ,including HPV typing and E6/E7 mRNA detection. Immunohistochemistry were used to determine CD47 expression , and histochemistry score(H⁃score) was used to quantify the levels of CD47. The correlation among HPV 16/18 , pathological grades and CD47 was analyzed.
Results:Bioinformatics tool analysis and immunohistochemistry showed that CD47 was up⁃regulated in HSIL and CC , associated with the prognosis of CC. The expression levels of CD47 in cervical tissues increased significantly after HPV 16/18 infection ( t = 2. 494 , P < 0. 05 ) , and in HPV 16/18 infected tissues , with the increase of pathological grades. The expression levels of CD47 significantly increased(F = 4. 351 , P < 0. 05 ; rs = 0. 278 , P < 0. 001) . The expression of CD47 in the E6/E7 mRNA( + ) was higher than that in the E6/E7 mRNA( - ) ( t = 5. 710 , P < 0. 000 1) , and the copies of E6/E7 were related to the degree of cervical lesions , and were positively correlated with the expression level of CD47 ( F = 15. 557 , P <0. 000 1 ; rs = 0. 649 , P < 0. 000 1) ( rs = 0. 73 , P < 0. 000 1) .
Conclusion:CD47 is overexpressed in HSIL and CC after HPV 16/18 infection. The expression levels of CD47 increases with the degree of cervical lesions , and is correlated with E6/E7 mRNA. CD47 may be a potential target for cervical disease progression and therapy.
- Full text:202407071002363263CD47的表达与宫颈病变程度的相关性分析_朱雨婷.pdf
