The influence of serum substituents on serum-free Vero cell conditioned culture media manufactured from Dulbecco's modified Eagle medium in mouse embryo culture.
10.5468/ogs.2013.56.5.320
- Author:
Jong Seon LEE
1
;
Ju Hwan KIM
;
Young Seok SEO
;
Jung Bo YANG
;
Yong Il KIM
;
Hye Jin KIM
;
Ki Hwan LEE
Author Information
1. Department of Obstetrics and Gynecology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea. oldfox@cnuh.co.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Coculture;
Mouse embryo;
Serum free culture media, Vero cells
- MeSH:
Animals;
Coculture Techniques;
Culture Media, Conditioned;
Eagles;
Embryonic Development;
Embryonic Structures;
Female;
Follicular Fluid;
Humans;
Mice;
Pregnancy;
Vero Cells
- From:Obstetrics & Gynecology Science
2013;56(5):320-329
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study was conducted to examine the influences of supplementation of the serum substituents and available period of serum-free Vero cell conditioned media (SF-VCM) manufactured from Dulbecco's modified Eagle medium cultured with Vero cells for in vitro development of mouse preimplantation embryos. METHODS: A total of 1,099 two-cell embryos collected from imprinting control region mice were cultured in SF-VCM with 10% and 20% human follicular fluid (hFF), serum substitute supplement (SSS), and serum protein substitute (SPS). Development of embryos was observed every 24 hours. Results between different groups were analyzed by chi-square test, and considered statistically significant when P-value was less than 0.05. RESULTS: The rates of embryonic development cultured in SF-VCM supplemented with serum substituents were significantly higher compare with serum-free group (P < 0.05). The rates of embryonic development after 48 hours (morula< or =) and 96 hours (blastocyst< or =) were significantly higher in 20% SSS and 10% SPS than in 20% hFF supplementation (P < 0.05). And the rates of embryonic development after 96 hours (hatching blastocyst< or =) were significantly higher in 10% SPS (94.5%) than in 20% SSS (82.6%) and 20% hFF supplementation (68.5%). The rates of embryonic development according to storage period of the SF-VCM supplemented with 10% SPS showed no significant difference between control, 2 weeks and 4 weeks group. However developmental rate in 6 weeks storage group was significantly lower than other groups. CONCLUSION: The rate of embryonic development after 96 hours (hatching blastocyst< or =) was significantly higher in SF-VCM supplemented with 10% SPS. And storage period of media up to 4 weeks did not affect on embryonic development.
