A neuron-specific marker in the cerebral cortex
10.3760/cma.j.issn.1671-8925.2011.10.007
- VernacularTitle:一种大脑皮层神经元特异性标记物的研究
- Author:
Chuan-Wei WANG
1
;
Jie GONG
;
Hong-Wei WANG
;
Li-Ping NING
;
Shu-Gan ZHU
;
Yu-Guang LIU
Author Information
1. 山东大学齐鲁医院
- Keywords:
Cerebral cortex;
Neuron;
Biological marker;
Immunofluorescence
- From:
Chinese Journal of Neuromedicine
2011;10(10):996-999
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the characteristics of a new neuron-specific marker in the cerebral cortex and its application method.Methods According to immunofluorescence principles,the neurons in the cerebral cortex cultured in vitro were stained by fluorescein isothiocyanate (FITC)-labeled Tet1; and FITC-Tet1 with NSE or GFAP was employed to determine whether the neurons can be marked and whether they can be distinguished from astrocytes; the conditions of application of FITC-Tet1 were quantified.The HEK and C6 cell lines were performed the above experiments as controls.Results The final concentration was the key factor,which indicated that when the FITC-Tet1 was about 30 μg/mL,the neuron marking ability was the best,and the astrocytes,HEK and C6 were not stained.Neither high (100μg/mL) or low (10μg/mL) levels of FITC-Tet1 were suitableforstainingneurons.Conclusion Tet1 can specifically combine with the neuron in the cerebral cortex,which may make it to be a new staining method or a targeting molecule to neurons in the central nervous system.
