Differential expression of FAM83A and β-catenin in cervical lesions
10.19405/j.cnki.issn1000-1492.2024.01.009
- Author:
Fangning Dong
1
;
Chong Lan
2
Author Information
1. Shenyang Women ’ s and Children ’ s Hospital，Postgraduate Training Base of Jinzhou Medical University， Shenyang 110011; Dept of Gynecology，Shenyang Women ’ s and Children ’ s Hospital，Shenyang 110011
2. Shenyang Women ’ s and Children ’ s Hospital，Postgraduate Training Base of Jinzhou Medical University， Shenyang 110011
- Publication Type:Journal Article
- Keywords:
FAM83A;
β-catenin;
high-grade squamous intraepithelial lesion;
low-grade squamous intraepithelial lesion;
cervical squamous cell carcinoma;
immunohistochemical
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):52-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the expression difference and potential clinical significance of 83 sequence similar member A (FAM83A) and β-catenin in cervical lesions．
Methods :ALCAN and GEPIA2. 0 online data- bases were used to analyze the difference of FAM83A expression in normal cervix and cervical squamous cell carci- noma ( CSCC) and the relationship between FAM83A expression and the prognosis of CSCC patients，LinkedOmics database was used to analyze FAM83A mRNA co-expression genes，and R language was used for KEGG enrichment analysis．Immunohistochemistry was used to detect FAM83A and β-catenin expression in 60 cases of normal cervix， 80 cases of low-grade squamous intraepithelial lesion ( LSIL) ，90 cases of high-grade squamous intraepithelial le- sion (HSIL) and 70 cases of cervical squamous cell carcinoma ( CSCC) ．The relationship between FAM83A ，β - catenin expression and clinicopathological features and the correlation between FAM83A and β-catenin expression were analyzed.
Results : UALCAN database analysis showed that FAM83A was highly expressed in CSCC tissues， and GEPIA 2. 0 database analysis suggested that those with high FAM83A expression had a poor prognosis．Linke- dOmics database performing KEGG enrichment analysis suggested that expression of FAM83A was positively correla- ted with aberrant activation of Wnt / β-catenin signaling pathway． The expression rate of FAM83A in CSCC was higher than that in LSIL and normal cervical tissues (P＜0. 001) ，but there was no significant difference compared with HSIL (P = 0. 401) ; the expression of FAM83A was not correlated with age (P = 0. 231) ，but was significant- ly different from the correlation with differentiation (P = 0. 001) and clinical stage (P = 0. 038) ．The abnormal ex- pression rate of β-catenin in CSCC was higher than that in LSIL and normal cervical tissues (P＜0. 001) ，but there was no significant difference compared with HSIL (P = 0. 734) ; the expression of β-catenin was not related to age (P = 0. 088) ，related to differentiation (P = 0. 001) and clinical stage (P＜0. 001) ，and FAM83A was positively correlated with β-catenin expression (P ＜0. 05 ) ．
Conclusion :FAM83A and β-catenin are highly expressed in both HSIL and CSCC tissues，and there is a positive correlation between the expression of FAM83A and β-catenin. The high expression of FAM83A has some correlation with the prognosis of CSCC patients and can be used as a po- tential marker to determine the prognosis of CSCC.
- Full text:2024062415033243657FAM83A与β-cate...在宫颈病变组织中的表达差异_董芳宁.pdf
