Acacetin protects against cerebral ischemia-reperfusion injury by regulating ROS/NLRP3 signaling pathway via autophagy
10.19845/j.cnki.zfysjjbzz.2023.0025
- VernacularTitle:刺槐素通过自噬调控ROS/NLRP3信号通路对脑缺血再灌注损伤发挥保护作用
- Author:
Juan BU
1
;
Xiaoshan NIU
1
Author Information
1. Center for Medical Research and Transformation,People Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China
- Publication Type:Journal Article
- Keywords:
Acacetin;
Oxygen-glucose deprivation/reperfusion (OGD/R);
Microglial cells;
NLRP3 inflammasome;
Autophagy
- From:
Journal of Apoplexy and Nervous Diseases
2023;40(2):99-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects of Acacetin on mouse microglial cells injured by oxygen-glucose deprivation/reperfusion (OGD/R) based on autophagy/ROS/NLRP3 inflammasomes signaling pathway.Methods Mouse microglial cells (BV2) were divided into normal control group,OGD model group and OGD+Acacetin group (10 μmol).After hypoxia for 6 hours and reoxygenation for 24 hours,the viability of BV2 cells was detected by 4-methylazolazolium blue (MTT) method.The death rate of cell was measured by lactate dehydrogenase (LDH) method.Reactive oxygen species (ROS) assay kit was used to measure the intracellular ROS level.The expression of LC3-Ⅱ,LC3-Ⅱ/LC3-Ⅰ,beclin-1,NLRP3,caspase-1 and IL-1β were analyzed by western blot.Results Acacetin increased the survival rate of microglia cells,and reduced the release of LDH and production of ROS.Acacetin also increased the expression of LC3-Ⅱ,and further to decrease the expression of NLRP3,caspase-1 and IL-1 β.Conclusions Acacetin protected against cerebral ischemia-reperfusion injury by attenuating the injury of microglia after OGD/R.Its protective mechanism may be related to inhibiting the production of ROS,activating autophagy,and inhibiting the NLRP3 inflammasome.
- Full text:2024061723044333198Acacetin protects against cerebral ischemia-reperfusion injury by regulating ROS_NLRP3 signaling pathway via autophagy.pdf
