Effect of selinexor combined with imatinib on proliferation and apoptosis of K562/G01 cells
- VernacularTitle:塞利尼索联合伊马替尼对K562/G01细胞的增殖及凋亡的影响
- Author:
Xiaojing HAO
1
;
Liangming MAO
Author Information
- Keywords: Selinexor; chronic myelogenous leukemia; K562/G01; Imatinib-resistant; apoptosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(3):405-410
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the effect of Selinexor(SEL)combined with Imatinib(IM)on the proliferation and apoptosis of Imatinib-resistant chronic myeloid leukemia K562/G01(KG)cells and explore the possible mechanisms.Methods K562 cells and KG cells were treated with SEL or IM respectively or in combination.Cells viability was examined by MTT assay.Apoptosis was assessed by flow cytometry.BCR-ABL mRNA was detected by RT-PCR.XPO1 was detected by Western blotting.Results IM and SEL both inhibited the proliferation of K562 cells and KG cells;half maximal inhibitory concentration(IC50)for 48 h was 0.16 μmol/L vs.6.48 μmol/L for IM and 132.0 nmol/L vs.275.9 nmol/L for SEL.Compared with SEL or IM alone,SEL combined with IM significantly inhibited the proliferation of KG cells(P＜0.05),induced KG cells apoptosis(P＜0.05),downregulated the levels of BCR-ABL mRNA(P＜0.05),and inhibited the expressions of XPO1 in KG cells(P＜0.05).Conclusion SEL combined with IM can synergistically inhibit the proliferation and induce apoptosis of KG cells,and then inhibit the expressions of BCR-ABL mRNA and XPO1 to exert an anti-leukemia effect.
