Capsular specific typing of multidrug-resistant Klebsiella pneumoniae isolated from clinical cases in Ramadi city of Iraq
- Author:
Hasan Falah Lahij
1
;
Safaa Abed lateef Almeani
2
Author Information
- Publication Type:Journal Article
- Keywords: Capsule; Iraq; Klebsiella pneumoniae; K2; MDR
- From:Malaysian Journal of Microbiology 2024;20(no.1):40-45
- CountryMalaysia
- Language:English
-
Abstract:
Aims:The increasing incidence of Klebsiella pneumoniae infections in the community and hospitals is a considerable health problem. This is due to the rising resistance of the bacteria to antibiotics, biofilm formation and the presence of a capsule. The aim of this study was to survey the most common capsular types in local isolates for the first time in Iraq on a molecular level.
Methodology and results:Seventy isolates were screened for multidrug resistance (MDR) using a standard test. Genomic DNA was extracted from all isolates and PCR was performed using a multiplex PCR assay to detect the capsular type genes for K1, K2, K5, K20, K54 and K57. Forty-eight (68.5%) isolates demonstrated resistance to at least one agent of three or more antimicrobial categories and were therefore considered as MDR isolates. Multiplex PCR showed that 16/48 (33.3%) of MDR isolates belonged to the K2 capsular type and two isolates belonged to the K57 capsular type. The other four capsular types were not detected.
Conclusion, significance and impact of study:The K2 capsular type was the most common capsular type among MDR K. pneumoniae isolates from urinary tract infections (UTI) in Ramadi, Iraq. Monitoring capsular type is essential in addition to monitoring antibiotic resistance, as highly resistant strains with hypervirulent types can be particularly dangerous. - Full text:20.2024my0006.pdf
