Application of DNA image cytometry ploidy analysis in pathological diagnosis of malignant pleural effusion
10.3760/cma.j.cn115355-20230925-00123-1
- VernacularTitle:细胞图像DNA倍体分析在恶性胸腔积液病理诊断中的应用
- Author:
Lingling LIU
1
;
Li LI
;
Cuiling WU
;
Lijuan HOU
;
Wenliang ZHENG
Author Information
1. 山西白求恩医院（山西医学科学院 同济山西医院） 山西医科大学第三医院病理科，太原 030032
- Keywords:
Pleural effusion, malignant;
DNA image cytometry ploidy analysis;
Liquid-based cytology;
Diagnosis
- From:
Cancer Research and Clinic
2024;36(2):128-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical application value of DNA image cytometry ploidy analysis (DNA-ICM) in the pathological diagnosis of malignant pleural effusion.Methods:A retrospective case series study was conducted. The clinical data of 101 patients with pleural effusion from October to December 2021 in Shanxi Bethune Hospital were retrospectively analyzed. Liquid-based cytology (LBC) and DNA-ICM were performed on pleural effusion specimens. The sensitivity and specificity of the two methods were compared with the clinical diagnosis, imaging, biopsy, and follow-up results of the patients.Results:Among the pleural effusions of 101 patients, 39 were malignant pleural effusions and 62 were benign pleural effusions. The sensitivity of LBC and DNA-ICM in diagnosing malignant tumor cells in pleural effusions was 74.7% and 94.9%, respectively, and the specificity was 98.4% and 83.9%, respectively; the combination of the two had an increased diagnostic positivity rate compared with that of LBC alone [36.6% (37/101) vs. 28.7% (29/101)]. Seven cases with positive DNA-ICM but negative LBC result were followed up, and 1 case was diagnosed as small cell lung cancer. Conclusions:DNA-ICM can effectively improve the positive cytology detection rate of pleural effusion, and the combined detection of DNA-ICM and LBC can reduce the underdiagnosis rate of cytology, which is of great clinical value in the pathological diagnosis of malignant pleural effusion.
