Establishment of HPLC fingerprints and determination of eleven constituents for Qiangxin Mixture

VernacularTitle:强心合剂HPLC指纹图谱建立及11种成分测定
Author: Zi-Wei GAO ¹ ; Lei WU ; Xin-Rong LIN ; Xiao-Hu CHEN ; Wei-Ya PU
Author Information

1. 南京中医药大学附属医院,江苏南京 210029;南京中医药大学第一临床医学院,江苏南京 210029
Keywords: Qiangxin Mixture; HPLC fingerprints; chemical constituents
From: Chinese Traditional Patent Medicine 2023;45(12):3895-3901
CountryChina
Language:Chinese
Abstract: AIM To establish the HPLC fingerprints for Qiangxin Mixture,and to determine the contents of quercetin-3-O-β-D glucose 7-O-β-D gentil disglycoside(QGG),sinarin thiocyanate,erucinic acid,macrocetin,calycosin-7-glucoside,plananthine D,calycosin,baicalin,pilonidin,atracolactone Ⅲ and methylophiopogon dihydrogen isoflavone B.METHODS The analysis of methanol extract of this drug was performed on a 40℃thermostatic Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.01 mol/L phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS There were twenty common peaks in the fingerprints for fifteen batches of samples with the similarities of more than 0.930.Eleven constituents showed good linear relationships within their own ranges(r≥0.999 1),whose average recoveries were 98.47%-102.85%with the RSDs of 0.76%-1.74%.CONCLUSION This accurate and stable method can be used for the quality control of Qiangxin Mixture.