Discussion on the Mechanism of Jianpi Xiaoai Prescription in the Treatment of Colorectal Cancer Based on UPLC-Q-TOF-MS Combined with Network Pharmacology and Experimental Verification
10.19879/j.cnki.1005-5304.202307304
- VernacularTitle:基于UPLC-Q-TOF-MS结合网络药理学与实验验证探讨健脾消癌方治疗结直肠癌作用机制
- Author:
Li WANG
1
;
Cheng SONG
;
Wei TANG
Author Information
1. 湖南中医药大学,湖南 长沙 410208;湖南省肿瘤医院,湖南 长沙 410031
- Keywords:
Jianpi Xiaoai Prescription;
network pharmacology;
colorectal cancer;
UPLC-Q-TOF-MS
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(3):26-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the active components of Jianpi Xiaoai Prescription and its mechanism in the treatment of colorectal cancer(CRC).Methods UPLC-Q-TOF-MS technology was used to identify the chemical components of Jianpi Xiaoai Prescription;Active components and target proteins were screened through TCMSP and SwissTargetPrediction databases;STRING database was used for protein interaction analysis;The GeneCards database was used to screen CRC-related targets;DAVID database was used for GO and KEGG pathway enrichment analysis;Molecular docking verification of main active components and key targets was conducted.A rat model of CRC was constructed,and the effect of Jianpi Xiaoai Prescription on key targets of CRC-related pathways in rats was verified by Western blot.Results Totally 35 active components of Jianpi Xiaoai Prescription,617 potential targets,and 325 intersected with CRC-related targets were obtained.The enrichment analysis of active component targets showed that the main enrichments were PI3K-Akt signaling pathway,EGFR tyrosine kinase inhibitors signaling pathway,T cell receptor signaling pathway,VEGF signaling pathway and other related pathways.13 key active components and 6 key proteins of EGFR,SRC,CA2,CA12,CYP19A1 and MAPK3 were obtained in Jianpi Xiaoai Prescription.The results of Western blot experiments showed that Jianpi Xiaoai Prescription could significantly reduce the protein expression levels of SRC and EGFR in rat CRC samples.Conclusion The active component in Jianpi Xiaoai Prescription may exert anti CRC cell proliferation,invasion,and metastasis effects by regulating EGFR,SRC with multiple targets and pathways.
