Effects of histidine 74 in rabies virus matrix protein on mitochondrial localization
10.3969/j.issn.1002-2694.2023.00.147
- VernacularTitle:狂犬病病毒基质蛋白74位氨基酸位点对其线粒体定位的影响
- Author:
Xi ZHANG
1
;
Shu-Jie WANG
;
Jun ZHOU
;
Chen GAO
;
Qin LIU
;
Hong-Jing ZHOU
;
Yun-Bin XU
Author Information
1. 遵义医科大学,贵州省普通高等学校传染病与生物安全特色重点实验室,遵义 563000
- Keywords:
rabies virus;
M protein;
mitochondrial localization
- From:
Chinese Journal of Zoonoses
2023;39(12):1139-1145
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the effect of histidine(H)at position 74 on the localization of full-length viral matrix protein(M)in mitochondria.A eukaryotic expression vector expressing the N-terminus or C-terminus of wild-type M protein or its histidine 74 mutant protein(H→P,H74P)fused with V5(tag protein)-APEX2(modified ascorbate peroxidase)or A-PEX2-V5 was constructed and transfected into 293T cells.Fluorescence staining and laser confocal microscopy were used to an-alyze the effect of histidine 74 mutation on the localization of full-length M protein in mitochondria.Immunoblotting showed that the eukaryotic expression vector expressed the fusion protein normally.Fluorescence staining indicated that the H→P mu-tation at position 74 did not affect the ability of the full-length viral M protein to localize to mitochondria.Our findings regard-ing the effect of histidine 74 on the mitochondrial localization of full-length virus M protein provide a foundation for future study of the biological function of M protein and the replication mechanism of rabies virus.
