Comparison of the infectivity and stability of Asian versus African lineage Zika virus in glial cells
10.3969/j.issn.1002-2694.2023.00.127
- VernacularTitle:亚洲系与非洲系寨卡病毒株对神经胶质细胞感染性及稳定性差异研究
- Author:
Yu-Feng YU
1
;
Zhen-Rui HE
;
Shao-Jun QIAO
;
Xiu-Ling FAN
;
Shan WANG
;
Jian WANG
Author Information
1. 功能蛋白山西省重点实验室,山西医科大学基础医学院,太原 030001
- Keywords:
Zika virus;
glial cells;
infectivity;
stability
- From:
Chinese Journal of Zoonoses
2023;39(11):1080-1086
- CountryChina
- Language:Chinese
-
Abstract:
Infection of pregnant women by Asian lineage Zika virus(ZIKV)is more likely to cause microcephaly in infants than African lineage ZIKV.To further clarify the differences in infectivity and stability between Asian and African lineage ZIKV,we selected Asian lineage SZ01 and African lineage MR766 strains for study in glial cells.First,we examined the differ-ence in infectivity between SZ01 and MR766 on U251 and U87 astrocytes,and T98G glioblastoma cells,by using CCK8 assays.Subsequently,we examined the tolerance of SZ01 and MR766 to 37 ℃ and 40 ℃ in the free and infected cell states,by using qRT-PCR or viral plaque assays.Finally,we examined the effect of repeated freezing and thawing on the stability of SZ01 and MR766 with viral plaque assays.ZIKV had higher infectivity in U251 and U87 than T98G,and SZ01 was more infectious to as-trocytes than MR766.SZ01 tolerated 40 ℃ better than MR766 in the free state.SZ01 proliferated faster than MR766 in glial cells at 40 ℃.The tolerance of SZ01 to repeated freezing and thawing was higher than that of MR766.The above findings sug-gest that the infectivity and stability of Asian lineage ZIKV are significantly higher than those of African lineage ZIKV,possi-bly because of its persistent infection and pathogenicity.
