Study on effect of Wumei Pill on apoptosis of colon cells in rats with ulcerative colitis and its mechanism by regulating miR-146a
10.3969/j.issn.1000-484X.2024.03.019
- VernacularTitle:乌梅丸通过调控miR-146a对溃疡性结肠炎大鼠结肠细胞凋亡的影响及机制研究
- Author:
Xue YANG
1
;
Lin CHU
Author Information
1. 泰州市中医院药学部,泰州 225300
- Keywords:
Wumei Pill;
miR-146a;
Ulcerative colitis;
Colon cells;
Proliferation;
Apoptosis
- From:
Chinese Journal of Immunology
2024;40(3):551-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effect of Wumei Pill on colon cell apoptosis in ulcerative colitis(UC)rats by regulating miR-146a and its mechanism.Methods:A total of 50 SD rats were selected,with 10 rats in each group,and divided into control group,model group,Wumei Pill low,medium and high doses groups.Transfected with anti-miR-NC,anti-miR-146a,miR-NC,miR-146a as anti-miR-NC group,anti-miR-146a group,Wumei Pill+miR-NC group,Wumei Pill+miR-146a group.CCK-8 was used to de-tect cell proliferation;flow cytometry was used to detect cell apoptosis;RT-qPCR was used to detect cell miR-146a expression;ELI-SA was used to detect cell IL-1β and IL-13 contents;Western blot was used to detect expressions of B-cell lymphoma 2(Bcl-2)and Bax proteins.Results:Compared with control group,cell survival rate,Bcl-2 protein expression in model group were decreased,while apoptosis rate,Bax protein expression,IL-1β,IL-13 contents and miR-146a expression were increased(P<0.05).Compared with model group,Wumei Pill significantly increased cell survival rate and Bcl-2 protein expression,decreased cell apoptosis rate,Bax protein expression,IL-1β,IL-13 contents and miR-146a expression(P<0.05).Inhibition of miR-146a increased cell survival rate,Bcl-2 protein expression,decreased cell apoptosis rate,IL-1β,IL-13 contents and Bax protein expression(P<0.05).Overexpression of miR-146a reversed effects of Wumei Pills on proliferation and apoptosis of colon cells in UC rats.Conclusion:Wumei Pill can reduce apoptosis of colon cells in UC rats by up-regulating expression of miR-146a.
