Effect of TRB3 on the Function of Gastric Cancer Cells by Regulating TGF-β1/Smad3/PAI-1 Axis
10.11969/j.issn.1673-548X.2023.12.020
- VernacularTitle:TRB3调控TGF-β1/Smad3/PAI-1轴对胃癌细胞功能的影响
- Author:
Yu YE
1
;
Xuefeng YIN
Author Information
1. 310003 浙江省中西医结合医院(杭州市红十字会医院)普外科
- Keywords:
Gastric cancer;
TRB3;
Proliferation;
TGF-β1/Smad3/PAI-1 axis
- From:
Journal of Medical Research
2023;52(12):93-98
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of TRB3 on gastric cancer cells function by regulating TGF-β1/Smad3/PAI-1 axis.Methods The expression of TRB3 protein in gastric cancer cell lines(AGS,HGC-27,NCI-N87,SNU5 and MKN45)and normal human gastric mucosal cells(GES-1)was detected by Western blot.Knockdown of TRB3 in gastric cancer cell AGS,CCK-8,fluores-cenceactivated cell sorter(FACS),Transwell assay,scratch assay and Western blot were used to detect cell proliferation,apoptosis,in-vasion,migration and the changes of TGF-β1/Smad3/PAI-1 signal pathway proteins.On the basis of knockdown of TRB3,TGF-β1 treated gastric cancer cell AGS,and the above experiments were repeated again.Results Compared with GES-1 cells,the expression of TRB3 protein increased in gastric cancer cell lines.the proliferation ability and the viability of cells(52％±12％,P＜0.001)were decreased;migration ability and cell healing rate(24％±6％,P＜0.001)were decreased;the invasive ability and the number of inva-sive cells(107±23,P＜0.001)were decreased;TGF-β1/Smad3/PAI-1 pathway was inhibited,and the expression of TGF-β1,p-SMAD3 and PAI-1 were decreased;the apoptosis and the proportion of apoptotic cells(17％±3％,P＜0.001)were increased.On the basis of knockdown of TRB3,TGF-β1 was used to treated gastric cancer cells AGS,the cell viability(73％±10％,P＜0.01)was increased;the migration ability and cell healing rate(63％±8％,P＜0.01)were increased;the number of invasive cells(252±20,P＜0.01)was increased;cell apoptosis and the percentage of apoptosis(12％±2％,P＜0.01)was reduced.Conclusion TRB3 pro-motes the development of gastric cancer by activating of TGF-β1/Smad3/PAI-1 signaling pathway.
