Gemcitabine-induced Exosomes Derived from Pancreatic Cancer Cells Promote the Proliferation and Invasion of Pancreatic Cancer Cells
10.11969/j.issn.1673-548X.2023.12.016
- VernacularTitle:吉西他滨诱导的胰腺癌细胞外泌体促进胰腺癌细胞的增殖和侵袭
- Author:
Baoqing XU
1
;
Xiaojuan OUYANG
;
Xuzhou WANG
Author Information
1. 350025 福州,中国人民解放军联勤保障部队第九○○医院病理科
- Keywords:
Pancreatic cancer;
Exosomes;
Gemcitabine;
Proliferation;
Invasion
- From:
Journal of Medical Research
2023;52(12):71-75,82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of exosomes derived from pancreatic cancer cells treated with or without gemcitabine on the proliferation and invasion of pancreatic cancer cells.Methods Exosomes were extracted from supernatant of pancreatic cancer cells treated with or without gemcitabine.Transmission electron microscopy,nanoparticle tracking analysis and Western blot method were used to identify exosomes and the concentration and size of exosomes were determined.Incubation of exosomes with pancreatic cancer cells was performed to detect the proliferation and invasion of pancreatic cancer cells,and Western blot method was used to detect the expression of CD147.Results Exosome-specific markers were highly expressed in extracted exosomes.There was no significant difference of concen-tration and size of exosomes derived from pancreatic cancer cells treated with or without gemcitabine.Incubation with exosomes derived from pancreatic cancer cells promoted the proliferation and invasion of pancreatic cancer cells,and incubation with exosomes derived from pancreatic cancer cells treated with gemcitabine promoted the proliferation and invasion further,and the expression level of CD 147 was up-regulated significantly in gemcitabine-induced exosomes.Conclusion Gemcitabine-induced exosomes derived from pancreatic cancer cells can promote the proliferation and invasion of pancreatic cancer cells.
