Effect of long-chain non-coding ribonucleic acid HAGLR targeting micro ribo-nucleic acid-625-5p on lipopolysaccharide-induced apoptosis and inflammatory factor expression in retinal pigment epithelial cells
10.13389/j.cnki.rao.2024.0035
- VernacularTitle:LncRNA HAGLR靶向miR-625-5p对脂多糖诱导的视网膜色素上皮细胞凋亡和炎症因子表达的影响
- Author:
Jing LI
1
;
Weijuan ZENG
Author Information
1. 457000 河南省濮阳市,濮阳医学高等专科学校
- Keywords:
long-chain non-coding ribonucleic acid HAGLR;
micro ribonucleic acid-625-5p;
lipopolysaccharide;
reti-nal pigment epithelial cells;
apoptosis;
inflammation
- From:
Recent Advances in Ophthalmology
2024;44(3):178-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether long-chain non-coding ribonucleic acid HAGLR(LncRNA HAGLR)can affect lipopolysaccharide(LPS)-induced apoptosis and expression of inflammatory factors of retinal pigment epithelium(RPE)cells by targeted regulation of the expression of micro ribonucleic acid-625-5p(miR-625-5p),so as to lay an experi-mental foundation for revealing the mechanism of retinopathy.Methods LPS-induced human retinal pigment epithelial(ARPE-19)cells were set as the LPS group,and normally cultured ARPE-19 cells were assigned to the Con group.Quanti-tative real-time polymerase chain reaction was used to detect the expression levels of LncRNA HAGLR and miR-625-5p.Based on different transfection reagents,the cells were divided into the LPS+sh-NC group,LPS+sh-HAGLR group,LPS+miR-NC group,LPS+miR-625-5p group,LPS+sh-HAGLR+anti-miR-NC group,and LPS+sh-HAGLR+anti-miR-625-5p group.Flow cytometry was used to detect apoptosis rate;enzyme-linked immunosorbent assay was used to detect the lev-els of interleukin-6(IL-6)and interleukin-1β(IL-1β);the targeting relationship between LncRNA HAGLR and miR-625-5p was verified;Western blot was used to detect the protein levels of activated cysteinyl aspartate specific proteinase 3 and 9(cleaved-Caspase 3 and cleaved-Caspase 9).Results Compared with the Con group,the LPS group showed an increase in the expression level of LncRNA HAGLR and a decrease in the expression level of miR-625-5p(both P＜0.05),and there were increases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05).Compared with the LPS+sh-NC group,the LPS+sh-HAGLR group showed decreases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05);LncRNA HAGLR could negatively regulate the expression level of miR-625-5p(P＜0.05).Compared with the LPS+miR-NC group,the LPS+miR-625-5p group showed an increase in the expression level of miR-625-5p and decreases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05).Compared with the LPS+sh-HAGLR+anti-miR-NC group,the LPS+sh-HAGLR+anti-miR-625-5p group showed a decrease in the expression level of miR-625-5p and increases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05).Conclusion Interference on LncRNA HAGLR expression can realize the targeted regulation of miR-625-5p expression to inhibit the apoptosis and inflammatory factor expression,reducing LPS-induced injury of ARPE-19 cells.
