Study on the Relationship between CLDN10 and CSPG4 Levels Expression of Tissue and Clinicopathological Characteristics and Prognosis in Non-small Cell Lung Cancer
10.3969/j.issn.1671-7414.2024.02.009
- VernacularTitle:非小细胞肺癌组织中CLDN10和CSPG4水平表达与临床病理特征及预后的关系研究
- Author:
Min CHEN
1
;
Yahui SHEN
Author Information
1. 南京医科大学附属泰州市人民医院呼吸与危重症医学科,江苏泰州 225300
- Keywords:
non-small cell lung cancer;
claudin 10;
chondroitin sulfate proteoglycan 4
- From:
Journal of Modern Laboratory Medicine
2024;39(2):46-50,74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the expression of claudin 10(CLDN10)and chondroitin sulfate proteoglycan 4(CSPG4)of tissue and clinicopathologic characteristics and prognosis in non-small cell lung cancer(NSCLC).Methods A total of 136 NSCLC patients who underwent surgery in Taizhou People's Hospital,Nanjing Medical University from January 2017 to April 2018 were randomly selected as the study subjects,and these subjects were grouped into survival group(n=63)and death group(n=69)based on follow-up outcomes(survival or death).Specimens of cancer tissue and adjacent tissues excised during surgery were collected from 136 patients.Real-time fluorescence quantitative PCR(RT-qPCR)was applied to detect the expression levels of CLDN10 and CSPG4 in cancer tissue and adjacent tissues.Based on the average expression levels of CLDN10 and CSPG4 in cancer tissues of NSCLC patients,they were grouped into CLDN10 high expression group(n=66),CLDN10 low expression group(n=70)and CSPG4 high expression group(n=71),CSPG4 low expression group(n=65).Multivariate COX regression analysis was used to determine the prognostic factors of NSCLC patients.Kaplan-Meier survival curve was applied to analyze the relationship between the expression levels of CLDN10 and CSPG4 in NSCLC patients and their prognosis.Results The expression level of CLDN10(0.96±0.25)in NSCLC tissues was lower than that in adjacent tissue(1.73±0.42),and the expression level of CSPG4 in NSCLC tissues(1.80±0.46)was higher than that in adjacent tissues(1.04±0.27),with statistical significance(t=18.372,16.617,all P<0.05).Compared with the survival group,the proportion of low undifferentiated,lymph node metastasis and CSPG4 expression of cancer tissues in the death group were higher,and the CLDN10 expression level of cancer tissues in the death group was lower,with statistical significance(t/χ2=8.463,7.423,11.696,6.426,all P<0.05).The five-year survival rate of the high expression group of CLDN10(58.46%)was higher than that of the low expression group(37.31%),and the five-year survival rate of the low expression group of CSPG4(55.56%)was higher than that of the high expression group(40.58%),and the differences were statistically significant(χ2=7.848,4.018,all P<0.05).Multivariate COX analysis results showed that low expression of CLDN10,high expression of CSPG4,low differentiation degree,and lymph node metastasis were all risk factors affecting the prognosis of NSCLC patients(HR=1.362,1.368,1.335,1.314,all P<0.05).Conclusion The expression level of CLDN10 was low and CSPG4 was high in cancer tissues of NSCLC patients,which was closely related to the development and prognosis of NSCLC.
