The changing levels of GluR2 phosphorylation in oligodendrocyte precursor cells in mice model of ischaemic hypoxia
10.16557/j.cnki.1000-7547.2023.06.008
- VernacularTitle:缺血缺氧模型小鼠少突胶质前体细胞GluR2磷酸化水平的变化
- Author:
Fei ZHEN
1
;
Ruiqin YAO
;
Zhen QIU
Author Information
1. 淮安市洪泽区人民医院病理科,淮安 223110
- Keywords:
glutamate receptor 2(GluR2);
hypoxic ischemic brain injury(HIBI);
preterm white matter injury(PWMI);
oligodendrocyte precursor cells(OPCs);
mouse
- From:
Chinese Journal of Neuroanatomy
2023;39(6):671-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of phosphorylated glutamate receptor 2[p-GluR2(S880)]in oligodendrocyte precursor cells(OPCs)of mice model of hypoxic ischemic brain injury(HIBI).Methods:The HIBI model of C57BL/6 neonatal mice was established by right common carotid artery ligation and hypoxia for 90 min.The anxiety-like behavior of the mice was evaluated by elevated plus maze(EPM)and open field test(OFT).The expres-sion of p-GluR2(S880),oligodendrocyte marker 4(O4)and myelin basic protein(MBP)in brain tissue of HIBI model mice was detected by immunofluorescence staining.What's more,the expression levels of p-GluR2(S880)and MBP were detected by Western Blot.Results:Compared with sham operation group,there were significant anxiety-like behaviors 90 days after HIBI operation(P＜0.05).The expression of MBP protein decreased significantly in 14 and 28 days after HIBI operation.The expression of p-GluR2(S880)protein was up-regulated at all time points after HIBI op-eration(P＜0.05),and the number of O4 and p-GluR2(S880)double positive cells in brain tissue of HIBI group was significantly increased(P＜0.05).Conclusion:The up-regulation of p-GluR2(S880)expression in OPCs may lead to myelination disorder in HIBI model mice.
