Effects of Ferroptosis Inhibitor KIF20A on Biological Behavior and Ferroptosis of Esophageal Carcinoma Cells
10.12259/j.issn.2095-610X.S20240207
- VernacularTitle:铁死亡抑制因子KIF20A对食管癌细胞生物学行为及铁死亡的影响
- Author:
Maimaitizunong REZEYE
1
;
Xiujuan LI
;
Ling LIU
;
Hui LI
Author Information
1. 新疆医科大学基础医学院生物化学与分子生物学教研室,新疆 乌鲁木齐 830000
- Keywords:
Ferroptosis;
KIF20A;
ESCC;
RSL3
- From:
Journal of Kunming Medical University
2024;45(2):49-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of KIF20A in ESCC and its effect on Eca109 cell biological behavior and ferroptosis.Methods The expression of KIF20A in ESCC was predicted by bioinformatics analysis.KIF20A knockdown/overexpression Eca109 cell line was constructed,Experimental groups:control group,KIF20A knockdown group,KIF20A overexpression group,and the effects of KIF20A on the proliferation,invasion,and migration of Eca109 cells were detected by CCK-8,Transwell invasion assay and cell scratch assay.On this basis,the ferroptosis model induced by RSL3 was established,and GSH and MDA contents were detected.Results Compared with the control group,the cell proliferation activity of the KIF20A knockdown group was significantly decreased(P<0.05),and that of the KIF20A overexpression group was significantly increased(P<0.05).Compared with the control group,the invasion and migration ability of the KIF20A knockdown group was decreased(P<0.05),and the invasion and migration ability of the KIF20A overexpression group was increased(P<0.05).After RSL3 induction,the GSH content in the cell lysate of the KIF20A knockdown group was lower than that of the control group(P<0.05),and the MDA content was higher than that of the control group(P<0.05).Conclusion KIF20A is highly expressed in ESCC and plays a role in promoting proliferation,invasion,migration and inhibiting ferroptosis in Eca109 cells.
