Jiegu Qili Capsules Promotes Fracture Healing of Radius in Rats by Activating the BMP/Smad Signaling Pathway
10.13359/j.cnki.gzxbtcm.2024.04.029
- VernacularTitle:接骨七厘胶囊通过激活BMP/Smad信号通路促进大鼠桡骨骨折愈合
- Author:
Yan-Tao JIA
1
;
Xiao-Long LU
Author Information
1. 湖南中医药大学,湖南长沙 410208;湖南中医药大学第二附属医院,湖南长沙 410208
- Keywords:
Jiegu Qili Capsules;
fracture;
radius;
BMP/Smad signaling pathway;
SaOS-2 cells;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(4):1011-1018
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the improvement effect of Jiegu Qili Capsules on the fracture healing of radius in rats by activating the bone morphogenetic protein(BMP)/Smad signaling pathway.Methods(1)A rat model of radius fracture was constructed,and the serum levels of alkaline phosphatase(ALP),calcium(Ca)and phosphorus were detected,and the pathological changes in the fracture gap was observed.(2)Human osteosarcoma SaOS-2 cells were used to measure ALP activity and mineralization level.The quantitative real-time polymerase chain reactionn(qRT-PCR)method was used to detect the cellular osteogenesis-related genes ALP,collagen I(COL-I),ornithine transcarbamylase(OTC),Osterix,osteoblastin(OPN),Runt-related transcription factor 2(RUNX2)and BMP2.The expression of key proteins in BMP/Smad signal pathway was detected by Western Blot.Results Jiegu Qili Capsules effectively promoted fracture healing of radius in rats,enhance ALP activity,increase Ca and P levels Jiegu Qili Capsules stimulate the formation of mineralized nodules in SaOS-2 cells in rats.,and promoted the expression levels of COL-I,OTC,Osterix,BMP2 and OPN in SaOS-2 cells in a dose-dependent manner.Jiegu Qili Capsules up-regulated the levels of Smad1/5 phosphorylation of the BMP/Smad signaling pathway in SaOS-2 cells,as well as the levels of BMP2 and RUNX2.Noggin,an inhibitor of the BMP/Smad signaling pathway,inhibited osteogenic differentiation of SaOS-2 cells induced by Jiegu Qili Capsules.Conclusion Jiegu Qili Capsules can promote fracture healing by activating the BMP/Smad signaling pathway to increase the expression of osteogenesis-related genes.
