Expression of CD70 in hepatocellular carcinoma and its impact on tumor microenvironment based on TCGA database
10.3760/cma.j.cn115396-20230904-00047
- VernacularTitle:基于TCGA数据库分析CD70在肝细胞癌中的表达及其对肿瘤微环境的影响
- Author:
Zhihua LONG
1
;
Qing XU
Author Information
1. 中国康复研究中心 北京博爱医院普外科，北京 100068
- Keywords:
Hepatocellular carcinoma;
Antigens, CD70;
Antigens, CD27;
Immune checkpoint;
Tumor microenvironment
- From:
International Journal of Surgery
2023;50(10):676-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression level of CD70 and receptor CD27 in hepatocellular carcinoma (HCC) and their relationship with clinicopathological features and prognosis, and to explore the possible role of CD70 in the tumor microenvironment.Methods:Download the RNA sequencing data related to HCC from The Cancer Genome Atlas (TCGA), and analyze the expression differences of CD70 and CD27 between HCC and normal tissues, and their relationship with clinicopathological features. Kaplan-Meier survival curve was used to compare the survival rate of patients with different expressions of CD70 and CD27. The correlation between CD70 and other genes that affect the expression of tumor microenvironment was analyzed.Results:The data of 371 HCC tumor samples and 50 normal tissue samples were obtained by TCGA database. The expression of CD70 in HCC tumor tissues was significantly higher than that in normal tissues. There was no significant correlation between CD70 expression and age, gender, tumor stage, distant metastasis and survival ( P>0.05). The expression of CD27 in HCC tumor tissues was not significantly different from that in normal tissues, but the overall survival rate of patients with high CD27 expression was significantly higher than that of patients with low CD27 expression. The expression of CD70 in HCC tumor tissue was significantly positively correlated with the expressions of IL-12, FOXP3, CD25, CD39, CD27, PD-1, PD-L1, LAG3, CTLA4, Tim3, VEGF, IDO, CXCR4 and CCL2 ( P<0.05, | r|>0.3). Conclusion:CD70 is abnormally expressed in HCC. Although CD70 expression is not significantly associated with pathological staging and survival in HCC patients, it is related to the immunosuppressive tumor microenvironment, which may be one of the mechanisms of immune escape in HCC.
