Expression and clinical significance of maternal embryo leucine zipper kinase in renal clear cell carcinoma
10.3760/cma.j.cn121382-20230617-00509
- VernacularTitle:母体胚胎亮氨酸拉链激酶在肾透明细胞癌中的表达及临床意义
- Author:
Fen LIU
1
;
Shibin LI
;
Xiaoshi LI
Author Information
1. 新乡市中心医院，新乡医学院第四临床学院病理科，新乡 453000
- Keywords:
Maternal embryonic leucine zipper kinase;
Renal clear cell carcinoma;
Cell proliferation
- From:
International Journal of Biomedical Engineering
2023;46(5):433-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of maternal embryonic leucine zipper kinase (MELK) in renal clear cell carcinoma and its relationship with clinical and pathological characteristics of the patients with renal clear cell carcinoma, and to evaluate its effect on the proliferation of renal clear cell carcinoma.Methods:The expression of MELK in renal clear cell carcinoma and its relationship with the overall survival of patients was analyzed using the gene expression profiling interactive analysis (GEPIA) online database. Cancer tissue samples from 77 patients with renal clear cell carcinoma treated at Xinxiang Central Hospital were collected, all of which have been confirmed as renal clear cell carcinoma through immunohistochemistry experiments. The relationship between the expression of MELK in renal clear cell carcinoma tissue and the clinical and pathological characteristics of the patients was analyzed. The effect of MELK on the proliferation of renal clear cell carcinoma cells was explored through cloning experiments and CCK-8 assay.Results:Information from the GEPIA database revealed that the expression level of the MELK gene was upregulated in renal clear cell carcinoma compared with normal tissues. The clinicopathological analysis results showed that MELK expression was significantly correlated with tumor size and diameter of renal clear cell carcinoma ( P < 0.05), and had no statistically significant correlation with patient age, gender, or tumor differentiation. The results of the clonogenic assay and the CCK-8 assay showed that MELK expression promoted the proliferation of renal clear cell carcinoma cells (all P < 0.05). Conclusions:MELK promotes the proliferation of kidney cancer cells, which may provide a new therapeutic strategy for the treatment of renal clear cell carcinoma.
