Establishment and Application of a 42-plex Microhaplotype Assay in Forensic Genetics
10.12116/j.issn.1004-5619.2023.530803
- VernacularTitle:42个微单倍型复合检测体系的构建及法医学应用
- Author:
Peng YUN
1
;
An-Qi CHEN
;
Li-Qin CHEN
;
Cheng-Tao LI
Author Information
1. 内蒙古医科大学法医学教研室,内蒙古 呼和浩特 010030;司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴定专业技术服务平台,上海 200063
- Keywords:
forensic genetics;
microhaplotype;
next-generation sequencing;
mixture;
individual identifica-tion
- From:
Journal of Forensic Medicine
2024;40(1):50-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and forensically verify a 42 microhaplotypes(mircohaps,MHs)mul-tiplex assay system based on next-generation sequencing(NGS),and to explore the application value of this system in the practice of forensic genetics.Methods A total of 42 highly polymorphic MHs were selected from previous studies,and sequenced by the MiSeq FGxTM platform to verify the repeata-bility,sensitivity,specificity,stability,and mixture analysis ability of the detection system.Through population genetic investigation of 102 unrelated Chinese Han individuals in Liyang City,Jiangsu Province,China,the application value of this system in forensic genetics was evaluated.Results The sequencing repeatability of the 42-plex MHs assay was 100%and the sensitivity was as low as 0.062 5 ng.The system had the ability to withstand the interference of indigo(≤2 500 ng/μL),humic acid(≤9 ng/μL),hemoglobin(≤20 μmol),and urea(≤200 ng/μL)and to detect mixtures of 2 people(1∶19),3 people(1∶1∶9)and 4 people(1∶1∶1∶9).Based on 102 individual data,the combined power of discrimination and the combined power of exclusion were 1-3.45×10-30 and 1-3.77×10-11,respectively,and the average effect value of alleles was 2.899.Conclusion The 42-plex MHs assay was successfully established in this study and this system has high repeatability and sensitivity,good anti-jamming ability and mixture analysis ability.The 42 MHs are highly polymorphism and have good application value in individual identification and paternity testing.
