KLF7 Affects Development of Broiler Adipose Tissue by Inhibiting Hypoxia￣Inducible Factor 1 Alpha Transcription
10.13865/j.cnki.cjbmb.2021.01.1334
- Author:
Zhao JIN
1
;
Shu-Li SHAO
1
;
Ming TAN
1
;
Jin-Wei LI
1
;
Bing-Hao JIA
1
;
Wei-Yu WANG
1
;
Yao ZHOU
1
;
Ting-Ting CUI
1
;
Ying-Ning SUN
1
Author Information
1. Department of Biotechnology, College of Life Science and Agriculture Forestry, Qiqihar University
- Publication Type:Journal Article
- Keywords:
adipose tissue;
chicken;
hypoxia-inducible factor 1 alpha (HIF1α);
Krüppel-like factor 7 (KLF7);
transcription
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(3):363-371
- CountryChina
- Language:Chinese
-
Abstract:
Krüppel-like factor 7 (KLF7) is a negative regulator of adipogenesis, whereas hypoxia-inducible factor 1 (HIF1) promotes anoxic-induced adipose tissue development in mammals. Our previous ChIP-seq analysis showed that one of the KLF7 binding peaks was present upstream of hypoxia-inducible factor 1 alpha (HIF1α), indicating that KLF7 may regulate HIF1α transcription. For this purpose, ChIP-PCR was used to verify ChIP-seq results, which showed that KLF7 directly bound to the HIF1α upstream region. Dual luciferase reporter and qRT-PCR results showed that KLF7 overexpression significantly decreased the luciferase reporter activity of HIF1α (- 4 432/- 4 182) (P < 0. 01) and inhibited HIF1α expression. After the deletion of KLF7 binding motif “TGCGCAGCAA” (- 4 300/-4 290) predicted by bioinformatics, the luciferase reporter activity of HIF1α (-4 432/-4 182) was significantly enhanced compared with wild-type plasmid (P<0. 01). Furthermore, Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) at the age of 1-7 weeks from the 19
