Analysis of the Interaction between Eglin C and 2709 Alkaline Protease and Establishment of an Affinity Purification Method
10.13865/j.cnki.cjbmb.2021.02.1617
- Author:
Yang-Xuan WEN
1
;
Gui-Xu ZHOU
1
;
Ya-Wei SHI
1
Author Information
1. Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University
- Publication Type:Journal Article
- Keywords:
affinity purification;
alkaline protease;
eglin C;
interaction
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(4):487-494
- CountryChina
- Language:Chinese
-
Abstract:
Eglin C is a small heat-stabilized protein from leeches. It belongs to the potato chymotrypsin inhibitor family, and can inhibit elastase, subtilisin, cathepsin, α-lytic protease and chymotrypsin etc. However, purification of protease by eglin C has not been studied. In this paper, the cDNA sequences encoding eglin C and its mutants were chemically synthesized and cloned into the prokaryotic expression vector pQE30. His
