Knockdown of Homeobox D12 Inhibits 7 Factor-Induced Somatic Cell Reprogramming
10.13865/j.cnki.cjbmb.2021.06.1176
- Author:
Shi-Cai FANG
1
;
Yi HUANG
1
;
Shi-Cai FANG
2
;
Yi HUANG
2
;
Bo WANG
2
;
Chen LI
2
;
Jin MING
2
;
Duan-Qing PEI
2
;
Bo WANG
3
;
Guo-Qing ZHAO
3
;
Chun-Yang DONG
4
;
Chuang LI
4
Author Information
1. Department of Biology, GMU-GIBH Joint School of Life Science, Guangzhou Medical University
2. Department of Cell Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
3. Cell Fate Lineage Center of Bio-land Laboratory, Guangzhou Regenerative Medicine and Health Guangdong Laboratory
4. Department of Medical Biotechnology, School of Life Sciences, University of Science and Technology of China
- Publication Type:Journal Article
- Keywords:
embryonic stem cells (ESCs);
homeobox D12 (Hoxd12);
induced pluripotent stem cells (iPSCs);
proliferation;
somatic cell reprogramming
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(9):1188-1196
- CountryChina
- Language:Chinese
-
Abstract:
Differentiated cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by overexpressing defined transcription factors. The process of reprogramming requires the interaction of various transcription factors to regulate the transformation of cell fate. Hoxd12 (Homeobox D12) is one of the transcription factors regulating the embryonic development of vertebrates, and it plays an outstanding role in the development of the limb, body axis formation, and cell signal transduction. However, any roles of Hoxd12 may play in the somatic cell reprogramming and the pluripotency of embryonic stem cells (ESCs) have not been reported. In this study, we firstly used 7 factors (Sall4-Esrrb-Jdp2-Glis1-Mkk6-Nanog-Kdm2b) and Yamanaka factors (Oct4-Klf4-Sox2) as the research model, combined with RNA interference (shRNA) and gene overexpression, to explore the mechanism of Hoxd12 in somatic cell reprogramming. Moreover, we used CRISPR/Cas9 gene editing to construct Hoxd12 knockout embryonic stem cell lines, and combined embryoid body formation (EB) and RNA sequencing (RNA-seq) to explore the function of Hoxd12 in the pluripotency of ESCs. The conclusions are as follows: (1) Knocking down of Hoxd12 inhibits 7 factor-induced reprogramming (
