Hsa_circ_0087354 Regulates Proliferation and Redox State via hsa-miR-199-3p / SLC7A11 in MG-63 Cells
10.13865/j.cnki.cjbmb.2022.01.1588
- Author:
Bao-Lian ZHENG
1
;
Jia-Xuan HE
1
;
Pei-Qi LIANG
1
;
Dan LI
1
;
Xiao-Chuan LIU
1
;
Jing-Ying ZHANG
1
Author Information
1. Key Laboratory of 3D Printing for Stomatology, The First Dongguan Affiliated Hospital, Guangdong Medical University
- Publication Type:Journal Article
- Keywords:
hsa-miR-199-3p;
hsa_circ_0087354;
nuclear factor erythroid-2 relatedfactor 2 (Nrf2);
oxidative stress;
solute carrier family 7 member 11 (SLC7A11)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(3):308-319
- CountryChina
- Language:Chinese
-
Abstract:
Circular RNAs (circRNAs),a kind of novel non-coding RNAs, have been shown to play animportant role in cellular redox reactions. In the previous study, we found that hsa_circ_0087354 wasclosely related to the cellular redox state by real-time PCR. After overexpression of hsa_circ_0087354, the relative expression of ROS1 were decreased significantly (P < 0. 01), while the levels of SOD1 wereincreased significantly (P < 0. 05) . The activities of SOD and Gpx as well as GSH concentration weresignificantly increased (P < 0. 01), and cell proliferation was promoted in cells (P < 0. 05) . Bioinformatics analysis predicted that there were binding sites between hsa-miR-199-3p and hsa_circ_0087354 as well as solute carrier family 7 member 11 (SLC7A11), which might have a targetedregulatory relationship. Dual luciferase reporter assay confirmed the targeted regulatory relationshipbetween hsa-miR-199-3p with hsa_circ_0087354, and SLC7A11. Overexpressed hsa_circ_0087354 plasmid and ctrl plasmid were constructed, hsa-miR-199a-3p, hsa-miR-199b-3p and hsa-miR-NC mimicswere synthesized. Real-time PCR analysis showed that the relative expression of hsa-miR-199-3p was observably decreased (P < 0.01), while the relative expression of SLC7A11 in cells was dramaticallyincreased after transfection of has_circ_0087354 plasmid (P < 0.05) . After transfection with hsa-miR-199-3p, the relative expressions of SLC7A11 were markedly decreased (P < 0.001) . The activities ofSOD and Gpx, GSH concentration (P<0.01), and cell proliferation rate (P < 0.05) were significantlyreduced. In conclusion, hsa_circ_0087354 could enhance the expression of SLC7A11, promote theproliferation of cells and reduce the oxidative stress by adsorption of hsa-miR-199-3p.
