Macrophage Activation Induced by TMAO through CD147 / MMPs
10.13865/j.cnki.cjbmb.2021.10.1350
- Author:
Qi-Lin LIU
1
;
Tao ZHANG
1
;
Yun-Tian LI
1
;
Yi XUE
2
;
Xiao-Hui LAI
2
;
Da-Yong DU
2
;
Yang LIU
2
;
Yun-Tian LI
2
Author Information
1. Second Clinical Medical College of Southern Medical University
2. PLA 305 Hospital
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
extracellular matrix metalloproteinase inducer CD147;
matrix metalloproteinases;
mousemacrophages RAW264. 7;
plaque stability;
trimethylamine N-oxide
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(1):91-96
- CountryChina
- Language:Chinese
-
Abstract:
Trimethylamine N-oxide (TMAO), a metabolite of intestinal flora, can promote Atherosclerosis (AS) in various ways. Current studies have found that it has a close relationship with plaque stability in clinical practice, but its molecular mechanism remains unclear at present. Extracellular matrix metalloproteinase inducers (CD147) / matrix metalloproteinases (MMPs) regulate a signal pathway highly related to plaque stability, which can promote plaque instability and lead to cardiovascular adverse events by weakening the thickness of the fibrous cap. Therefore, in this study, the mouse macrophageRAW264. 7 was stimulated by TMAO to establish a cell model to observe the effects on CD147, MMP2, and MMP9, and the CD147 gene silencing model was further constructed by using the siRNA transfection method to explore the interaction between CD147 and MMP2 and MMP9. Rt-qPCR and Western blotting results showed that there was no significant change in the gene expression level of CD147 in mouse macrophage RAW264. 7, but significantly increased in protein levels (P < 0. 05), while MMP2 andMMP9 were increased in mRNA and protein levels (P<0. 05). The expression of CD147, MMP2, andMMP9 was significantly inhibited in CD147 siRNA transfected cells (P<0. 05). In conclusion, TMAO significantly increases the expression of MMP2 and MMP9 in mouse macrophages RAW264. 7, and this effect may be partially realized through the CD147/ MMP pathway.
