Perilla frutescens PfFUL Regulates the Flowering and Silique Development in Transgenic Arabidopsis thaliana
10.13865/j.cnki.cjbmb.2023.06.1001
- Author:
Xi GUO
1
;
Qi-Feng WANG
1
;
Hua-Xiang XU
1
;
Jie YU
1
;
Hui-Min SHI
1
;
Xiao-Yan FENG
1
;
Tao ZHANG
1
Author Information
1. College of Life Sciences, Chongqing Normal University
- Publication Type:Journal Article
- Keywords:
flowering;
FRUITFULL;
Perilla frutescens;
silique development
- From:
Chinese Journal of Biochemistry and Molecular Biology
2023;39(8):1133-1142
- CountryChina
- Language:Chinese
-
Abstract:
The FRUITFULL (FUL) gene belongs to the AP1/ FUL subfamily of the plant MADS-box family and has functions in regulating flowering time, floral meristem differentiation and fruit development. PfFUL gene sequence was derived from the perilla transcriptome data, and the basic physicochemical properties of PfFUL were analyzed by bioinformatics methods. Evolutionary relationships of PfFUL with other species of FUL were analyzed by phylogenetic tree. Plant expression vector 35S::PfFUL was constructed and used to transform wild type Col-0 and mutant ful-7 Arabidopsis to obtain overexpression 35S::PfFUL/ Col-0 and complemented mutation 35S::PfFUL / ful-7 plants respectively. Comparative phenotypic analysis was performed to preliminarily clarify the function of PfFUL gene in plant flowering and fruit development. The functions of the PfFUL gene during flowering and angular fruit development of the plants were initially clarified. The CDS of PfFUL gene is 738 bp and encodes 245 amino acids. Phylogenetic tree showed that the perilla PfFUL was closely related to Solanum lycopersicum, Salvia splendens and Salvia hispanica, but far related to Arabidopsis thaliana, Nicotiana tabacum and Vitis vinifera. Compared to Col-0 and ful-7, the transgenic plants showed early flowering (P<0. 05), and the silique length of 35S::PfFUL / ful-7 plants reverted to the wild-type phenotype (P>0. 05), and the amount of wrinkled seed was significantly reduced (P<0. 01). In addition, phenotypic observations revealed that the transgenic plants also exhibited increased internode length and narrowed and curled cauline leaves. In conclusion, this study confirmed that the PfFUL gene regulates early flowering and fruit development in plants and participates in nutritional growth.
