Detection of intestinal microbial changes in rotenone-induced Parkinson' s disease model mice
10.16098/j.issn.0529-1356.2020.04.006
- Author:
Qiu-Qin HAN
1
;
Guo-Qing WAN
1
;
Xue-Feng GU
1
Author Information
1. Research Department, Affiliated Zhoupu Hospital, Shanghai University of Medicine and Health Sciences
- Publication Type:Journal Article
- Keywords:
16S rRNA;
Intestinal microbe;
Mouse;
Open field experiment;
Rotenone;
[Keywords] Parkinson' s disease
- From:
Acta Anatomica Sinica
2020;51(4):507-512
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective To investigate the changes of intestinal microbes in rotenone-induced Parkinson' s disease (PD) mice based on 16S rRNA gene sequencing. Methods Fourteen 8-week-old male C57BL/6J mice were randomly divided into two groups: 6 mice in the control group and 8 mice in the model group. The model mice were injected subcutaneously with rotenone (3 mg/kg) for 5 weeks, and the bod)' weight was measured once a week. After 5 weeks, behavioral tests were perfonned, including the rotating rod test and the open field test. The contents of the tract were used for intestinal microbial detection analysis. Results After 5 weeks of rotenone treatment, the weight of PD mice was significantly lower than that of the control mice(P<0. 0 5) . The movement time of the PD model mice in the rotating rod (P<0. 0001) and the total distance of movement in the open field(P<0. 05) were significantly lower than that of the control mice. In addition, the intestinal microbial diversity of the PD model mice did not change(P > 0. 05), but the microbial species showed significant differences. Among them, the PD mice showed a significant decrease in the intestinal Turicibacter (P < 0 . 0 1), a significant increase in norank f Lachnospiraceae (P < 0. 01), a significant decrease in norank_f Erysipelotrichaceae(P<0. 01), and a significant increase in Lachnoclostridium{ P<0. 0 1) . Conclusion Intestinal microbes in PD mice are disordered, and these intestinal flora ma)' be involved in the development of dyskinesia in PD mice.
