Protective effect of astragaloside on renal injury induced by radiation through toll-like receptor 4/nuclear factor kappa B pathway
10.16098/j.issn.0529-1356.2021.04.018
- Author:
Yu-Qin XU
1
;
Yan-Ping DING
1
;
Shu-Ning LIU
1
;
Jie KANG
1
;
Bao-Ping SHAO
2
Author Information
1. School of Life Science, Northwest Normal University
2. School of Life Science, Lanzhou University
- Publication Type:Journal Article
- Keywords:
Astragaloside IV;
Immunohistochemistry;
Immunoinflammatory response;
Kidney injury;
Mouse;
Nuclear factor kappa B;
Radiation;
Toll-like receptor 4
- From:
Acta Anatomica Sinica
2021;52(4):621-627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether astragaloside IV (AS-IV) can protect radiation-induced kidney injury through toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB)-mediated signaling pathway. Methods The mice were randomly divided into normal control group, DMSO solvent group, irradiation group (IR), IR + AS-IV 20 mg/kg group and IR + AS-IV 40 mg/kg group. One month after intraperitoneal injection of AS-IV, the mice were irradiated with 8 Gy 60Coγ and to the content of serum creatinine (Cr) and uric acid (BUA) in serum were determined, HE and immunohistochemical staining, and expression of TLR4/NF-κB signaling pathway related protein in kidney were performed. Results Compared with the control group, the levels of Cr and BUA in the serum of the radiation group increased significantly (P<0.001), glomerular atrophy and tubular expansion, TLR4 and myeloid differentiation factor 88(MyD88) positive expression increased significantly (P< 0.001), and the expression of TLR4/NF-κB signaling pathway-related proteins[ TLR4, MyD88, NF-κB, interleukin-1 β (IL-Iβ), tumor necrosis factor α (TNF-α)] increased significantly (P< 0.01); AS-IV pretreatment can reduce the content of Cr and BUA in serum (P<0.05, P<0.01 or P<0.001), significantly improve the pathological response caused by radiation, and reduce the expression of TLR4/NF-κB signaling pathway-related proteins (P<0.05 or P<0.01). Conclusion AS-IV may down-regulate the release of inflammatory factors through the TLR4/NF-κB signaling pathway, thereby improving radiation-induced kidney injury in mice and playing a protective role.
