Relationship between DEAD-box helicase 5, transcription factor 12 and amyotrophic lateral sclerosis
10.16098/j.issn.0529-1356.2021.05.005
- Author:
Bao-Yong LIN
1
;
Jin-Chao XU
1
;
Han-Tao YING
1
;
Bao-Yong LIN
2
;
Jin-Chao XU
2
;
Han-Tao YING
2
;
Xin JIANG
2
;
Huan-Cai LIU
2
;
Qing WANG
2
;
Qiao-Zhen WANG
2
;
Yan-Chun CHEN
2
;
Yan-Chun CHEN
3
Author Information
1. Biotechnology Specialty, School of Life Science and Technology
2. Key Laboratory of Neurological Diseases and Regenerative Repair
3. Department of Histology and Embryology, Basic Medical College, Weifang Medical University
- Publication Type:Journal Article
- Keywords:
Amyotrophic lateral sclerosis;
Co-immunoprecipitation assay;
Hippocampus;
mouse DEAD-box helicase 5;
SODI-G93A transgenic mouse;
Transcription factor 12
- From:
Acta Anatomica Sinica
2021;52(5):698-705
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression of DEAO-box helicase 5(DDX5) and transcription factor 12(TCF12) with amyotrophic lateral sclerosis ( ALS ) hippocampal lesions by detecting the expressions and the interaction of DDX5 and TCF12 in the hippocampus of SOD1-G93A mutant ALS transgenic mice. Methods Forty- two pairs of SOD1-G93A mutant ALS transgenic mice and wild-type mice were divided into three groups at the age of 95 days (early onset stage), 108 days (middle onset stage) and 122 days (late onset stage). RT-PCR, Western blotting and double immunofluorescence labeled technique were used to detect the expressions of DDX5 and TCF12 in the hippocampus. Co-immunoprecipitation assasy was used to detect the interaction between DDX5 and TCF12. Results Compared with the wild-type mice of the same age, DDX5 and TCF12 mRNA in the hippocampus of SOD1-G93A mutant ALS transgenic mice were unchanged, but DDX5 and TCF12 protein were up-regulated significantly at day 95, 108 and 122. DDX5 and TCF12 positive cells were found in both DG area and hippocampus proper, and DDX5 and TCF12 were co-localized with neurons. The immunoreactivities of DDX5 and TCF12 in the hippocampus of SOD1-G93A mutant transgenic mice were elevated compared with wild-type mice at the same time point. Co-immunoprecipitation assasys confirmed that there existed interactions between DDX5 and TCF12 protein. Conclusion DDX5 and TCF12 protein are up-regulated in the hippocampal tissues of SOD1-G93A mutant ALS transgenic mice. The abnormal expressions of DDX5 and TCF12 are involved in the hippocampal lesions of ALS.
