Wnt7a / β-catenin / autoimmune regulator signaling pathway involved in the occurrence of type 1 diabetes
10.16098/j.issn.0529-1356.2022.04.008
- Author:
Yuan-Di LI
1
;
Jing-Yi MA
1
;
Jia-Shan YU
1
;
Ke-Ke HE
1
;
Ting-Hao WEN
1
;
Jie GAO
1
;
Hong LI
1
;
Min SU
1
;
Rong HU
1
;
Min SU
2
;
Rong HU
3
Author Information
1. Department of Histology and Embryology, Basic Medical College, Guizhou Medical University
2. National Joint Local Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Province Key Laboratory of Regenerative Medicine, Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences), Guizhou Medical University
3. Translational Medicine Research Center, Guizhou Medical University
- Publication Type:Journal Article
- Keywords:
Autoimmune regulator;
NOD / Ltj mouse;
Real-time PCR;
Type 1 diabetes;
Western blotting;
Wnt signaling pathway
- From:
Acta Anatomica Sinica
2022;53(4):453-460
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective To investigate the relationship between the expression of Wnt signaling pathway, autoimmune regulator (AIRE) and type 1 diabetes (T1D) tissue specific antigen (TSAs) insulin 2(Ins2) and glutamic acid decarboxybase(GAD67) in thymus and the occurrence of T1D in NOD/ Ltj mice with spontaneous type 1 diabetes (T1D). Methods Sixty female NOD/ Ltj mice were divided into three groups: 3 weeks group, 16 weeks non-onset group and 16 weeks onset group. Two consecutive non-fasting blood glucose levels ≥ 11. 1 mmol/ L were considered as the occurrence of T1D. Pancreatic HE staining was used to observe the occurrence of islet inflammation. Anti-Ins and CD45 immunohistochemical staining showed islet cells or infiltrating inflammatory cells. The protein levels and mRNA expressions of Wnt7a, -catenin, AIRE, Ins and GAD67 in thymus were detected by Western blotting and Real-time PCR. The proportion of T cells in thymus was analyzed by flow cytometry. Results 1. With the occurrence of T1D, the islet structure was destroyed, a large number of lymphocytes infiltrated, and the remaining islet cells were reduced. A large number of CD45
