Polymorphism of 5,10-methylenetetrahydrofolate reductase gene rsl801131 in Qinghai Han patients with hypertensive disorders complicating pregnancy
10.16098/j.issn.0529-1356.2022.05.008
- Author:
Ming YUAN
1
;
Qian DUAN
1
;
Wen-Jia LI
1
;
Ru WANG
1
;
Zhen WU
1
;
Jian-Hua LI
1
Author Information
1. Department of Human Anatomy, Medical College of Qinghai University, Basic Medicine Science
- Publication Type:Journal Article
- Keywords:
Genetic polymorphism;
Hypertensive disorders complicating pregnancy;
Methylenetetrahydrofolate reductase;
Pregnant woman;
Restriction fragment length polymorphism polymerase chain reaction
- From:
Acta Anatomica Sinica
2022;53(5):594-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the polymorphism of 5,10-methylenetetrahydrofolate reductase ( MTHFR) gene rs!801131 and hypertensive disorders complicating pregnancy ( HDCP ) in Qinghai Han nationality. Methods The polymorphism of MTHFR rsl801131 in 120 pregnant women with HDCP (HDCP group) and 120 normal pregnant women ( control group) were detected by restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) and verified by sequencing. Results The frequencies of AA, AC, and CC genotype of MTHFR gene in the HDCP group were 56. 67% , 32. 50% , and 10. 83% respectively, and those in the control group were 74.17%, 23.33% and 2. 50% respectively (P<0. 05, the distribution of genotype was different significantly between the two groups). The frequency of AA genotype of HDCP group (56. 67%) was lower than that of control group (74. 17%, P<0. 05) , the frequency of CC genotype of HDCP group ( 10. 83%) was higher than that of control group ( 2. 50% , P< 0. 05) , while there was no significant difference in the frequency of AC genotype between HDCP group and control group ( P<0. 05). The frequency distribution of alleles A and C of MTHFR rsl801131 polymorphism was significantly different between the HDCP group and the control group (P<0. 001) , and the frequency of allele C in the HDCP group was significantly higher than that in the control group (X2 = 12. 229, 0R=L 574, 95% C/= 1. 181-2. 099, P<0. 001). Conclusion The polymorphism of MTHFR rsl801131 is related to the occurrence of HDCP in Qinghai Han population. The C gene might be the susceptibility gene of HDCP, and CC genotype might be the susceptibility genotype of HDCP.
