Expression of elastin and collagen in rats with heart failure
10.16098/j.issn.0529-1356.2022.05.015
- Author:
Ke-Ke WANG
1
;
Xiang-Guang MENG
1
;
Qian WANG
1
;
Zhi-Kun GUO
1
;
Huan-Huan LUO
2
;
Zhi-Kun GUO
3
Author Information
1. Henan Key Laboratory of Arrhythmia, Zhengzhou No. 7 People ' S Hospital
2. School of Nursing, Xinxiang Collage
3. Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University
- Publication Type:Journal Article
- Keywords:
Collagen;
Elastin;
Heart failure;
Immunofluorescence;
Matrix metalloproteinase;
Rat;
Real-time PCR;
Western blotting
- From:
Acta Anatomica Sinica
2022;53(5):637-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of elastin and collagen in heart of rats with chronic heart failure and their related proteases. Methods Sprague-Dawley rat model of chronic heart failure was made by intraperitoneal injection of doxorubicin hydrochloride. Examination of cardiac function by echocardiography. ELISA and immunohistochemistry were used to detect brain natriuretic peptide ( BNP) , and Masson staining was used to detect myocardial fibrosis. The mRNA levels of elastin, I /IH collagen were detected by Real-time PCR and the expression levels of elastin, I/IH collagen, Matrix metalloproteinase (MMP)-2 and MMP-9 were detected by Western blotting. Results Compared with the control group, the heart function of rats in the model group decreased, the BNP content in serum increased, the expression of BNP in myocardial tissue was strongly positive, and the mRNA and protein expression of elastin decreased. Nevertheless, the mRNA and protein expression of I / IH collagen increased. At the same time, both MMP-2 and MMP-9 protein expression levels increased in model rat heart. Conclusion Heart failure leads to decreased expression of elastin, I /IH collagen expression increased, and the MMP-2 and MMP-9 may be involved in the regulation of this process.
