Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues
10.16098/j.issn.0529-1356.2022.06.002
- Author:
Ya-Nan FAN
1
;
Chao-Hong LI
1
;
Xiang-Lei JIA
1
;
Jia-Hao JIN
1
;
Yu-Zhen LIU
1
;
Ya-Nan FAN
2
Author Information
1. Henan Key Laboratory of Neural Regeneration, the First Affiliated Hospital of Xinxiang Medical University
2. Department of Clinical Laboratory, Zhengzhou Sixth People's Hospital
- Publication Type:Journal Article
- Keywords:
Chronic intermittent Irypoxia;
Rat;
Superior cervical ganglion;
Western blotting;
β-site amyloid precursor protein cleaving enzyme 1
- From:
Acta Anatomica Sinica
2022;53(6):698-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACEl) in rat superior cervical ganglion and the effect of chronic intermittent frypoxia (CIH) on BACEl level. Methods The expression and distribution of BACEl in superior cervical ganglion were detected by RT-PCR, Western blotting and immunohistochemistry. Totally 16 male SD rats were randomly divided into control group and CIH group, 8 rats in each group. After 2 weeks of modeling, the effect of CIH on BACEl and peroxisome proliferators activated receptor gamma coactivator 1 alpha (PGC-la) mRNA level was detected by RT-PCR. Results BACEl was expressed in rat superior cervical ganglion, and mainly distributed in satellite glial cells and nerve fibers, but not in blood vessels, neurons and small intesely fluorecent(SIF) cells. CIH down-regulated BACEl mRNA level, but up-regulated PGC-la mRNA level ( P < 0.01). Conclusion BACEl is located in satellite glial cells and nerve fibers in the superior cervical ganglion of rats. The decreased level of BACEl ma)' be involved in the regulation of CIH-induced synaptic plasticity of superior cervical ganglion.
