Spatial and temporal expression patterns of Atoh1 during mice cerebellar development
10.16098/j.issn.0529-1356.2023.02.002
- Author:
Sai-Sai XUE
1
;
Hai-Tao WU
1
;
Li-Ping CHEN
2
;
Yan WU
2
;
Hai-Tao WU
2
;
Ying LI
3
Author Information
1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao University
2. Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences
3. Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University
- Publication Type:Journal Article
- Keywords:
Atoh1;
Cerebellum;
Development;
Granule neuron precursor cell;
Mouse;
RNA scope
- From:
Acta Anatomica Sinica
2023;54(2):134-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigated the spatial and temporal expression of Atoh1 mRNA and protein level during the cerebellar development. Methods The frozen cerebellum sections of mice at different developmental stages were used to analyze the temporal and spatial expression patterns of Atoh1 mRNA by RNA scope technique, n= 3 for each group. Meanwhile, two reporter mice with different genetic modification method were utilized to analyze the spatial and temporal expression of Atoh1 at the protein level by immunofluorescence staining, n= 3 for each group. Results Atoh1 mRNA was highly expressed in the embryonic cerebellar rhombic lip (RL) and the external granule layer (EGL). At the protein level, staining result of Atoh1-3
