Erythrocyte membrane-associated protein inhibiting Th17 cell differentiation via IL-6 / STAT3 / ROR-γt signaling pathway in experimental autoimmune encephalomyelitis mice
10.16098/j.issn.0529-1356.2023.05.006
- Author:
Ke-Ke HE
1
;
Yuan-Di LI
1
;
Ting-Hao WEN
1
;
Jie ZHU
1
;
Jie GAO
1
;
Rong HU
1
;
Min SU
1
;
Min SU
2
;
Jie GAO
3
;
Rong HU
3
;
Feng HAN
4
Author Information
1. Department of Histology and Embryology, Basic Medical College, Guizhou Medical University
2. National Joint Local Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Province Key Laboratory of Regenerative Medicine, Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences), Guizhou Medical University
3. Translational Medicine Research Center, Guizhou Medical University
4. Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical University
- Publication Type:Journal Article
- Keywords:
Erythrocyte membrane-associated protein;
Experimental autoimmune encephalomyelitis;
Flow cytometry;
Interleukin-6;
Mouse;
Retinoid acid receptor related orphan receptor-γt;
Signal transducers and activators of transcription 3;
Thelper cell 17
- From:
Acta Anatomica Sinica
2023;54(5):538-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of exogenous and endogenous erythrocyte membrane-associated protein (ERMAP) on helper T cell 17 (Th17) cell differentiation through interleukin 6 / signal transducers and activators of transcription 3 / retionoid-related orphan nuclear receptor-γt(IL-6 / STAT3 / ROR-γt) signal pathway in the mouse model of experimental autoimmune encephalomyelitis (EAE) . Methods Using flow cytometry to verify the function of ERMAP-Ig fusion protein at different concentrations; Agarose gel electrophoresis was performed to identify ERMAP knockout mice. Flow cytometry was performed to detect the effect of ERMAP-Ig fusion protein on Th17 cell differentiation in vitro. Forty 6-week-old normal C57BL / 6 mice were randomly divided into 2 groups to establish EAE models, control-Ig and ERMAP-Ig groups, with 20 mice in each group; Clinical scores were recorded; Flow cytometry was performed to detect Th17 cell differentiation in EAE mice in vivo. Forty 6-week-old identified wild-type and ERMAP knockout mice were divided into 2 groups to establish EAE models. Identified wild-type and ERMAP knockout mice were divided into 2 groups to establish EAE models, ERMAP
