Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method
10.16098/j.issn.0529-1356.2024.01.017
- Author:
Yona-Ming HAO
1
;
Han-Jun PEI
1
;
Li LI
1
;
Zhe ZHAO
1
;
Lei GUO
2
;
Cheng-Hui ZHOU
3
Author Information
1. Department of Cardiology, The First Affil. Hosp. of Baotou Med. College of Inner Mongolia University of Science and Technology
2. Inner Mongolia Erdos City Dharat Banner People1 S Hospital
3. Anesthesia Center, Fuwai Hospital, Chinese Academy of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Echocardiography;
Modified abdominal aortic coarctation method;
Myocardial hypertrophy;
Rat;
Western blotting
- From:
Acta Anatomica Sinica
2024;55(1):120-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare effectiveness between the modified and traditional pressure-overload myocardial hypertrophy(POMH) model by abdominal aorta coarctation (AAC) method. Methods Totally 45 rats were divided into three groups(n = 15 per group), sham group, traditional group, and modified group. In the traditional group, the diameter ol the abdominal aorta was narrowed to 0. 70 mm through a midline incision for 4 weeks; in the modified group, the diameter of the abdominal aorta was narrowed above the left kidney to 0. 45 mm for 1 week, and then the narrowing was lifted postoperatively. The cardiac index, heart weight (HW) /body weight (BW) and left ventricular index, left ventricular weight (LVW)/BW were measured from the heart specimens, and the cross-sectional area of cardiac myocytes, myocardial collagen area, and myocardial collagen area Iraction were measured in the pathological sections by HE staining and Masson staining. Results Compared with the sham group, the differences in end-systolic interventricular septum thickness (IVSs), left ventricular end-systolic posterior wall thickness (LVPWs), HW/BW, LVW/BW, cardiomyocyte cross-sectional area, myocardial collagen area, myocardial collagen area fraction, and brain natriuretic peptide (BNP) expression levels were statistically significant (P<0. 05) in the modilied and traditional groups of rats. The differences in these indices were not statistically significant between the modified and traditional groups (P>0. 05). Conclusion The modified abdominal aortic constriction method used in this experiment is time-saving, stable, homogeneous and easy to replicate, and is a more ideal approach to establish a rat model of POMH.
