Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia
10.16098/j.issn.0529-1356.2024.01.001
- Author:
Xi-Xi WEI
1
;
Chao-Hong LI
1
;
Chen-Lu ZHAO
1
;
Jia-Ping TANG
1
;
Yu-Zhen LIU
1
;
Xi-Xi WEI
2
;
Chao-Hong LI
2
;
Chen-Lu ZHAO
2
;
Jia-Ping TANG
2
;
Yu-Zhen LIU
2
Author Information
1. Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xinxiang Medical University
2. Life Science Research Center, The First Affiliated Hospital of Xinxiang Medical University
- Publication Type:Journal Article
- Keywords:
Chronic intermittent hypoxia;
Group Ⅰ metabotropic glutamate receptor;
Hypertension;
Rat;
Superior cervical ganglion;
Western blotting
- From:
Acta Anatomica Sinica
2024;55(1):3-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression and localization of group Ⅰ metabotropic glutamate receptors (mGluR1/ 5) in rat superior cervical ganglion (SCG) and the effect of chronic intermittent hypoxia (CIH) on mGluR1/ 5 protein level. Methods Twelve male SD rats were randomly divided into control group(Ctrl)and CIH group(CIH), 6 rats in each group. After 6 weeks of modeling, the effect of CIH on mGluR1/ 5 protein level was detected by Western blotting, the expression and distribution of mGluR1/ 5 in SCG were detected by immunohistochemistry and double-immunofluorescent staining. Results mGluR1/ 5 was expressed in rat SCG. mGluR1 was distributed in neurons and small intensely fluorescent (SIF) cells, but not in satellite glial cells (SGCs), nerve fibers and blood vessels, whereas mGluR5 was mainly distributed in nerve fibers and a little in neurons, but not in SGCs, SIF cells and blood vessels. CIH increased the protein levels of mGluR1/ 5 (P<0. 01) in rat SCG. Conclusion Both mGluR1 and mGluR5 are expressed in the rat SCG, but their distribution are different, and the increased protein levels of both may be involved in CIH-induced hypertension.
