Retinal microstructure and developmental characteristics in Zebrafish
10.16098/j.issn.0529-1356.2024.01.015
- Author:
Li-Ping FENG
1
;
Jun-Yong WANG
1
;
Jin-Xing LIN
1
;
Yi-Lin XU
2
;
Xun CHEN
2
;
Xiao-Ying WANG
2
;
Yi-Lin XU
3
;
Xun CHEN
3
;
Xiao-Ying WANG
3
;
Yi-Lin XU
4
;
Xun CHEN
4
;
Da-Hai LIU
5
Author Information
1. Shanghai Laboratory Animal Research Center
2. Department of Ophthalmology, Eye Ear Nose and Throat Hospital, Fudan University
3. National Health Commission Key Laboratory of Myopia of Fudan University
4. Shanghai Research Center of Ophthalmology and Optometry
5. Shanghai BK / KY Biotechnology Co. Ltd
- Publication Type:Journal Article
- Keywords:
Cone cell;
Retina;
Rod cell;
Transmission electron microscopy;
Zebrafish
- From:
Acta Anatomica Sinica
2024;55(1):105-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the microscopic structure and morphological characteristics of Zebrafish eyeball and retina at different developmental stages, and to lay a foundation for visual research model. Methods Select eight groups of zebrafish at different ages, with six fish in each group, 48 fish in total. Optical microscopy and transmission electron microscopy were used to observe the eyeball structure of Zebrafish at different developmental stages, and the thickness of retinal each layer was measured to analyze the temporal and spatial development pattern. The morphological characteristics of various cells in the retina and the way of nerve connection were observed from the microscopic and ultrastructural aspects, especially the structural differences between rod cells and cone cells. Results The retina of Zebrafish can be divided into ten layers including retinal pigment epithelial layer, rod cells and cone cells layer, outer limiting membrane, outer nuclear layer, outer plexiform layer, inner nuclear layer, inner plexiform layer, ganglion cell layer, nerve fiber layer, inner limiting membrane. Rod cells had a smaller nucleus and a higher electron density than cone cells. Photoreceptor terminals were neatly arranged in the outer plexiform layer, forming neural connections with horizontal cells and bipolar cells, and several synaptic ribbons are clearly visible within them. In Zebrafish retina, ganglion cell layer and inner plexiform layer are the earliest developed. With the growth and development of Zebrafish, the thickness of rod cells and cone cells layer and retinal pigment epithelial layer gradually increases, and the retinal structure was basically developed in about 10 weeks. Conclusion The retinal structure of Zebrafish is typical, with obvious stratification and highly differentiated nerve cells. There are abundant neural connections in the outer plexiform layer. The ocular development characteristics of Zebrafish are similar to those of most mammals.
