Chronopharmacokinetics research of helicid and its metabolites

Author: Yuanwei JIA ¹ ; Jie SHEN ¹ ; Haitang XIE ¹ ; Bin YANG ¹ ; Xianghong LI ¹ ; Jiru CHU ² ; Dandan ZHENG ² ; Cuifeng ZHANG ³ ; Jinghui ZHAO ⁴
Author Information

1. Drug Clinical Evaluation Center of Anhui Province, Yijishan Hospital of Wannan Medical College
2. Department of Pharmacy, Yijishan Hospital of Wannan Medical College
3. Anesthesia Laboratory & Training Center of Wannan Medical College
4. Department of Cardiology, Baoding NO1 Hospital
Publication Type:Journal Article
Keywords: Absorption; Chronopharmacokinetics; Helicid; Metabolism; Metabolite
From: Chinese Journal of Clinical Pharmacology and Therapeutics 2021;26(9):986-994
CountryChina
Language:Chinese
Abstract: AIM: To study chronopharmacokinetics of helicid and its metabolites. METHODS: An HPLC-MS method for simultaneous determination of helicid and its three phase I metabolites were established and validated. At 8:00, 14:00 and 0:00, the rats were given helicid 50 mg/kg by gavage, respectively. Blood samples were collected from ophthalmic venous plexus. Then plasma concentration was measured. Pharmacokinetic behaviors of the original drug and its metabolites after administration at different time points were calculated and compared. RESULTS: This established HPLC-MS/MS method was successfully applied to simultaneous determination of helicid and its three metabolites in rat plasma after intragastric administration. Using AUC